Prepare 100 mL of 0.01 M acetate buffer, pH 3.80, from stock solutions of 0.1 M acetic acid and 0.02 M sodium hydroxide. pKa acetic acid = 4.76.
Prepare 100 mL of 0.01 M acetate buffer, pH 3.80, from stock solutions of 0.1 M acetic acid and 0.02 M sodium hydroxide. pKa acetic acid = 4.76.
a. Use the Henderson-Hasselbalch equation to calculate the volume of each stock solution needed.
pH = pKa + log [conjugate base] / [weak acid]
b. Check your calculations with other students. See the instructor if there is uncertainty.
c. Make the solution and check the pH of a portion of your buffer solution using the pH meter.
d. Calculate the exact volume of the 0.01 M acetate buffer required to make 100 mL of 0.0005 M acetate buffer.
e. Prepare this new buffer using the following equation to aid you in your calculations.
C1V1 = C2V2
d. Check the pH of this new buffer.
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