Before this experiment, Dr Merrick didn’t know whether the OBP that he cloned was a type of myoglobin or hemoglobin from mammoth. From his data, he is now quite sure that OBP cannot be myoglobin from mammoth. How does he know? Explain your reasoning.
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Before this experiment, Dr Merrick didn’t know whether the OBP that he cloned was a type of myoglobin or hemoglobin from mammoth. From his data, he is now quite sure that OBP cannot be myoglobin from mammoth. How does he know? Explain your reasoning.
![Question 1) Dr John Merrick is characterizing a protein from a gene that he cloned from a frozen mammoth
tissue. Since this protein has the ability to bind oxygen, Dr Merrick called the protein OBP (oxygen binding
protein). To characterize OBP, he decided to compare its 02 binding ability with that of elephant hemoglobin.
He did the experiment at normal pH=7 and at lower pH=6.5.
Elephant Hb (pH=7)
Mammoth OBР (рн-7)
100
Elephant Hb
(PH36.5)
75
Mammoth OBP
50
(pH=6.5)
25
10
20
30
40
02 [µM]*
saturation (%)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb38f11b4-2041-4b9a-99fb-b8e390e6e98c%2F3dcc2b4e-9a25-48fc-ad85-4587b2a84865%2Fsr0b9nl_processed.png&w=3840&q=75)
Proteins can be monomeric or oligomeric. Monomeric proteins have a single subunit (single polypeptide chain) while oligomeric proteins have multiple subunits(multiple polypeptide chains). Hemoglobin is an oligomeric protein as it is composed of 4 subunits while myoglobin is monomeric. Myoglobin have one binding site for oxygen while hemoglobin have 4 (one in each subunit)
The shape of the oxygen saturation curve (the graph given in question) can provide us with information on the binding kinetics and even make a calculated guess on whether the protein is oligomeric or monomeric. The 2 types of curves generally exhibited are hyperbolic and sigmoidal curves. If a protein exhibits cooperativity in binding its ligand, then the graph will always be sigmoidal.
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