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ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 µM ATP (1000 μL of each) Inhibitors Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations Final concentrations (in 250 μL) 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! 8 µg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml

ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 µM ATP (1000 μL of each) Inhibitors Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations Final concentrations (in 250 μL) 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! 8 µg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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CALCULATIONS OF THE SOLUTIONS FOR EXPERIMENT Substrates Stock concentations Succinate + glutamatate (G/S) 150 mM Glutamtate + 150 mM Succinate Malate + pyruvate (M/P) 30 mM Malate + 30 mM Pyruvate Inhibitors Substrates of ETC ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 μM ATP (1000 μL of each) Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! Final concentrations (in 250 μL) 8 μg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml
Transcribed Image Text:CALCULATIONS OF THE SOLUTIONS FOR EXPERIMENT Substrates Stock concentations Succinate + glutamatate (G/S) 150 mM Glutamtate + 150 mM Succinate Malate + pyruvate (M/P) 30 mM Malate + 30 mM Pyruvate Inhibitors Substrates of ETC ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 μM ATP (1000 μL of each) Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! Final concentrations (in 250 μL) 8 μg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml
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