At a steady state in the living cell, (ATP) = 10-3M and (ADP) = 10-4 M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 C and pH=7. a. Calculate K and rG for the reaction. b. Using the value of rG for the reaction ADP + Pi →ATP + H2O, together with the answer to part (a), calculate rG and K for the reaction: glycerol + phosphate→ glycerol-1-phosphate+ H2O

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At a steady state in the living cell, (ATP) = 10-3M and (ADP) = 10-4 M. The maximum
(equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 C
and pH=7.
a. Calculate K and rG for the reaction.
b. Using the value of rG for the reaction ADP + Pi →ATP + H2O, together with the answer to
part (a), calculate rG and K for the reaction: glycerol + phosphate→ glycerol-1-phosphate+ H2O

10) A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol-
1-phosphate by ATP:
Glyserol (G) +
Mg2+
a-glycerol kinase
ATP
glyserol-1-phosphate (G-1-P) + ADP
At a steady state in the living cell, (ATP) = 10³M_and (ADP) = 10ª M. The maximum
(equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C
and pH=7.
a. Calculate K and A,G° for the reaction.
b. Using the value of AG° for the reaction ADP + P; →ATP + H2O, together with the answer to
part (a), calculate A,G° and K for the reaction: glycerol + phosphate> glycerol-1-phosphate+ H2O
Transcribed Image Text:10) A key step in the biosynthesis of triglycerides (fats) is the conversion of glycerol to glycerol- 1-phosphate by ATP: Glyserol (G) + Mg2+ a-glycerol kinase ATP glyserol-1-phosphate (G-1-P) + ADP At a steady state in the living cell, (ATP) = 10³M_and (ADP) = 10ª M. The maximum (equilibrium) value of the ratio (glycerol-1-P)/(glycerol) is observed to be 770 at 25 °C and pH=7. a. Calculate K and A,G° for the reaction. b. Using the value of AG° for the reaction ADP + P; →ATP + H2O, together with the answer to part (a), calculate A,G° and K for the reaction: glycerol + phosphate> glycerol-1-phosphate+ H2O
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