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Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? what is missir int

Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? what is missir int

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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dy Tube No. Additives Pepsin BAPNA 3. Pepsin 4 Pepsin BAPNA 5. Pepsin Pepsin BAPNA water water pH 2.0 Buffer Boil first, BAPNA BAPNA pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 7.0 Buffer pH 9.0 buffer Incubation Condition 37°C 37°C 37°C 37°C 37°C Incubate (all 60 min.) 37°C Predict results (before lab) Optical density Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? 4. Why is tube 4 described as "control" for this experiment on effect of pepsin? (hint -what is missing?) 5. Tubes 1 and 2 contained the same substances. Explain why their optical density measurements were different: 6. If you had not run the tube 2 and tube 3 samples, what argument could be made against the statement "Pepsin digests BAPNA"? 7. How did your predictions compare with actual results? Explain: Additional Activity: found at Mayo Clinic
Transcribed Image Text:dy Tube No. Additives Pepsin BAPNA 3. Pepsin 4 Pepsin BAPNA 5. Pepsin Pepsin BAPNA water water pH 2.0 Buffer Boil first, BAPNA BAPNA pH 2.0 Buffer pH 2.0 Buffer pH 2.0 Buffer pH 7.0 Buffer pH 9.0 buffer Incubation Condition 37°C 37°C 37°C 37°C 37°C Incubate (all 60 min.) 37°C Predict results (before lab) Optical density Answer Questions about your results: 1. Which pH provided the highest pepsin activity? Is that what you expected, based on location of pepsin secreting chief cells in the gastric glands? 2. Would pepsin be active in the mouth? Explain your response: 3. What are the products of protein digestion (subunits of proteins)? 4. Why is tube 4 described as "control" for this experiment on effect of pepsin? (hint -what is missing?) 5. Tubes 1 and 2 contained the same substances. Explain why their optical density measurements were different: 6. If you had not run the tube 2 and tube 3 samples, what argument could be made against the statement "Pepsin digests BAPNA"? 7. How did your predictions compare with actual results? Explain: Additional Activity: found at Mayo Clinic
ト PEX Assessing Pepsin Digestion of Proleim Reset ActIVIty Experiment Accessibility 2 Add the substances indicated below to tubes 1 through 6. Tube 1: pepsin, BAPNA, pH 2.0 buffer Tube 2: pepsin, BAPNA, pH 2.0 buffer Tube 3: pepsin, deionized water, pH 2.0 buffer Tube 4: deionized water, BAPNA, pH 2.buffer Tube 5: pepsin, BAPNA, pH 7.0 buffer Tube 6: pepsin, BAPNA, pH 9.0 buffer To add a substance to a test tube, drag the dropper cap of the bottle on the solutions shelf to the test tube. Undo Reset Spectrophotometer (405nm) Analyze Deionized pH 7.0 Buffer pH 2.0 BAPNA 06 Hd Pepsin Buffer Water Buffer Optical Density 3. 4. Temperature (°C) Timer (min) 2 of 14 Next ALWAYS LEARNING prt sc delete 12 144 %24 4 backspace 6. 8. 5. R. P. 95
Transcribed Image Text:ト PEX Assessing Pepsin Digestion of Proleim Reset ActIVIty Experiment Accessibility 2 Add the substances indicated below to tubes 1 through 6. Tube 1: pepsin, BAPNA, pH 2.0 buffer Tube 2: pepsin, BAPNA, pH 2.0 buffer Tube 3: pepsin, deionized water, pH 2.0 buffer Tube 4: deionized water, BAPNA, pH 2.buffer Tube 5: pepsin, BAPNA, pH 7.0 buffer Tube 6: pepsin, BAPNA, pH 9.0 buffer To add a substance to a test tube, drag the dropper cap of the bottle on the solutions shelf to the test tube. Undo Reset Spectrophotometer (405nm) Analyze Deionized pH 7.0 Buffer pH 2.0 BAPNA 06 Hd Pepsin Buffer Water Buffer Optical Density 3. 4. Temperature (°C) Timer (min) 2 of 14 Next ALWAYS LEARNING prt sc delete 12 144 %24 4 backspace 6. 8. 5. R. P. 95
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