An investigator briefly incubates an actively respiring bacterial culture with [1-*C]glucose and isolates the glycolytic and citric acid cycle intermediates. Where is 1“C located in each of the intermediates listed? Consider only the initial incorporation of “C in the first pass of labeled glucose through the pathways.

An investigator briefly incubates an actively respiring bacterial culture with [1-*C]glucose and isolates the glycolytic and citric acid cycle intermediates. Where is 1“C located in each of the intermediates listed? Consider only the initial incorporation of “C in the first pass of labeled glucose through the pathways.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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An investigator briefly incubates an actively respiring bacterial culture with [1-C]glucose and isolates the glycolytic and citric
acid cycle intermediates. Where is 14Clocated in each of the intermediates listed? Consider only the initial incorporation of 14C
in the first pass of labeled glucose through the pathways.
C-1 fructose 1,6-bisphosphate
С-3
glyceraldehyde 3-phosphate
C-3
| phosphoenolpyruvate
С-2
Answer Bank
acetyl-CoA
C-3
C-4
C-2
С-1
C-2
citrate
C-2
a-ketoglutarate
C-2
сз охаlоасetate
Incorrect

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