An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below. Glycogen (mM) Product Formed Glycogen only (mM/min) Product Formed Glycogen and Inhibitor (mM/min) 0.5 22.6 15.9 1.0 32.1 24.9 1.5 38.1 28.2 2.0 41.2 35.1 2.5 44.7 40.0 3.0 48.5 43.3 1. How to draw Lineweaver-Burke Plots, and list step by steps of calculation 2. Determine the form of inhibition observed from these results, and explain your rationale for this form. It is uncompetitive inhibition because the Km decrease when ES complex bind to inhibitor 3. Determine the values for Km and Vmax from these results.
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We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
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The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
- An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below.
|
Glycogen (mM) |
Product Formed Glycogen only (mM/min) |
Product Formed Glycogen and Inhibitor (mM/min) |
|
0.5 |
22.6 |
15.9 |
|
1.0 |
32.1 |
24.9 |
|
1.5 |
38.1 |
28.2 |
|
2.0 |
41.2 |
35.1 |
|
2.5 |
44.7 |
40.0 |
|
3.0 |
48.5 |
43.3 |
1. How to draw Lineweaver-Burke Plots, and list step by steps of calculation
2. Determine the form of inhibition observed from these results, and explain your rationale for this form.
It is uncompetitive inhibition because the Km decrease when ES complex bind to inhibitor
3. Determine the values for Km and Vmax from these results.
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In my text book, three types of inhibitions:
1, competitive inhibition
2. non-competitive inhibition
3. uncompetitive inhibition
Reversible competitive inhibition is not listed on my text book although I understand your reasponing in your context.
In my understanding, when we draw LB, it is always draw reversible line. If LB is not required, can I say this is competitive inhibition?
what are the tips to memorize these three types of inhibition?
by Bartleby ExpertHow did you get 59.17 mM/min
what is the calculation for this result?
by Bartleby Expert
