An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel.  Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ul

Q: DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to…

A: A restriction enzyme, also known as restriction endonuclease is an enzyme that cleaves DNA into…

Q: After properly troubleshooting, you finally are able to visualize the gel. You obtained the image…

A: DNA extraction is a protocol of isolating genomic DNA from any cell type. Generally we use the…

Q: 6. Identify the DNA sequence below. Write the DNA sequence in 5' to 3' orientation.

A: The DNA (deoxyribonucleic acid) sequence is the order in which the nucleotide bases are arranged.…

Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…

A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.

Q: Compute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA…

A: The appropriate amount of TE buffer needed for DNA extraction depends on the specific protocol or…

Q: which sample shows the plasmid is fully digested

A: 50 bp ladder sequence : 500 bp 450 bp 400 bp 350 bp 300 bp 250 bp 200 bp 150 bp 100 bp 50 bp

Q: Horizontal sequence :RIVL Vertical sequence:FMK   Scoring rules: g/o = -3, g/e = -1, match or…

A: Substitution matrix is used to find the relationship between two evolutionary systems. Substitution…

Q: How much 6x loading dye should be added to 49 uL of DNA?  Report you answer in units of uL, with one…

A: The equation of dilution: M1xV1 = M2xV2 Where, M1 is the molar concentration of the stock solution.…

Q: (a) Complete the table, using a letter D, R or B, to show whether each statement applies to: DNA…

A: Genetic material is the hereditary substance in the cell. It carries all information specific to…

Q: You have run a gel with 5 uL of amplified DNA in addition to 1 uL of 6X loading dye. Comparing the…

A: Agarose Gel Electrophoresis (AGE), is an biochemistry technique which is used to estimate DNA…

Q: 2. Discuss the mechanism of ethidium bromide (EtBr) and why is it used in staining DNA during…

A: "Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Indicate true or false for the following statements   The glycerol used in the DNA loading dye…

A: Gel electrophoresis is a method that uses the electric current to push the molecules like DNA, and…

Q: Write a short discussion based on the result above.

A: The peak at 260nm is significantly important here. The value of 260/280 and 260/230 helps in…

Q: You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many…

A: DNA is a double-stranded helix where two strands of DNA are linked to each other via hydrogen…

Q: . Draw the melting curve for primer annealing, labeling the axes. Label the position of the…

A:

Q: The short DNA shown below is to be sequenced. Using your knowledge of how the Sanger method works,…

A: This scenario based on DNA sequencing. DNA sequencing is the process of determining the order of…