An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ul
An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ul
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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An AGE run was set at 100V for 30 min.
The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel.
Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ul
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