Procedure1 to 2% of agarose gel was prepared in the running buffer. Mixture was swirled and mixed. The agarose and buffer mixture were boiled and mixed well until the agarose was completely dissolved.The agarose was cooled on the benchtop or by incubation in a 65 °C water bath.The gel tray was placed into the casting apparatus. The appropriate comb was placed to create the wells. The molten agarose was poured into the gel mold.The agarose was allowed to set at room temperature. The comb was removed and the gel was placed in the gel box. The running buffer was added until it covered the surface of the gel. A loading dye was loaded and the serum protein samples to be separated carefully.The lid was replaced to the gel box. Ensure the leads were plugged into the correct slots in the power supply. The power was turned on and started the electrophoresis running until the dye was migrated to an appropriate distance.After electrophoresis, the gel was stained with Coomassie brilliant blue and detain with detaining solution. Write a discussion based on the result. Agarose Gel Electrphoresis of Serum ProteinsResults1 2 3 4 5 6 7 8 9 10 11 12WellsWellsChicken serumHuman serum3.Chicken albuminChicken serum7Horse serum8Human serum9.Chicken albumin10Chicken IgHuman IgG1112

Serum proteins are mainly Albumin and different classes (alpha, beta , gamma) of globulins. Albumins…

Answered: Agarose Gel Electrphoresis of Serum…

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

Related questions

Question

Procedure

1 to 2% of agarose gel was prepared in the running buffer. Mixture was swirled and mixed. The agarose and buffer mixture were boiled and mixed well until the agarose was completely dissolved.
The agarose was cooled on the benchtop or by incubation in a 65 °C water bath.
The gel tray was placed into the casting apparatus. The appropriate comb was placed to create the wells. The molten agarose was poured into the gel mold.
The agarose was allowed to set at room temperature. The comb was removed and the gel was placed in the gel box. The running buffer was added until it covered the surface of the gel. A loading dye was loaded and the serum protein samples to be separated carefully.
The lid was replaced to the gel box. Ensure the leads were plugged into the correct slots in the power supply. The power was turned on and started the electrophoresis running until the dye was migrated to an appropriate distance.
After electrophoresis, the gel was stained with Coomassie brilliant blue and detain with detaining solution.

Write a discussion based on the result.