The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.
The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 42SST
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Question
The chart below represents the results (absorbance readings) of your Bradford Assay. Use the data and graph to determine the dilution of each well and concentration (in mg/ml) of the sample in each well of the microtiter plate.
![1
2
3.
4
A
2.1
1.397
0.226
0.053
0.013
0.271
0.321
0.397
0.506
0.835
Row A = absorbance readings of a 10 fold serial dilution of protein standards
Row B = readings of a absorbance 10 fold serial dilution of protein sample
CONC (X)* ABS (Y)
200
0.271
Standard Curve
400
0.321
600
0.413
0.8
800
0.538
0.7
y = 0.0005x + 0.1373
1000
0.672
0.6
0.5
*Concentration is in mg/ml
0.4
0.3
0.2
0.1
200
400
600
800
1000
1200
Concentration in mg/ml
Absorbance @ 595 nm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F6a30a93f-6c60-46a0-ac01-a3ee4ceb08eb%2F374b5e8b-daaf-40be-be71-a199be3b0244%2F7ith4n8_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1
2
3.
4
A
2.1
1.397
0.226
0.053
0.013
0.271
0.321
0.397
0.506
0.835
Row A = absorbance readings of a 10 fold serial dilution of protein standards
Row B = readings of a absorbance 10 fold serial dilution of protein sample
CONC (X)* ABS (Y)
200
0.271
Standard Curve
400
0.321
600
0.413
0.8
800
0.538
0.7
y = 0.0005x + 0.1373
1000
0.672
0.6
0.5
*Concentration is in mg/ml
0.4
0.3
0.2
0.1
200
400
600
800
1000
1200
Concentration in mg/ml
Absorbance @ 595 nm
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