Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: Albumin
Provide the Experimental procedure of the following: Example: Ninhydrin Test -The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation. Test that needs Experimental procedure: 1. Heat denaturation Samples: Albumin, gelatin 2. Isoelectric Precipitation Samples: Casein 3. Concentrated Mineral Acids Samples: Albumin 4. Organic Solvents Samples: Albumin
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Provide the Experimental procedure of the following:
Example:
Ninhydrin Test
-The samples needed in this test were 1% casein solution, albumin and gelatin and the reagent used was ninhydrin (Triketohydrindenehydrate or Triketohydrin hydrate). Test tube, test tube stand, pippete, water bath, and spectrophotometer were the materials and equipment used. Ninhydrin reagent in 1-2 drops was added to 0.5 ml of the sample with care. It was mixed and heated to boiling for 1-2 minutes. Then, it was allowed to cool to record the observation.
Test that needs Experimental procedure:
1. Heat denaturation
Samples: Albumin, gelatin
2. Isoelectric Precipitation
Samples: Casein
3. Concentrated Mineral Acids
Samples: Albumin
4. Organic Solvents
Samples: Albumin
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