Millon-Nasse Test Mix 1 mL of the sample and 5 drops of Millon-Nasse reagent. Boil the content gently for 30 seconds. Cool in water. Add 2 drops of freshly prepared 1% NaNO2 and warm gently. Observe the color of the precipitate and/or the solution. Question 1. Will all amino acids and proteins give a positive reaction to this test? Question 2. What causes the color of the solution or precipitate?

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