MATERIALS: Potato Thermometer Test Tubes Bunsen burner and tripod set up Distilled water 0.1% Catechol solution (in amber bottle) Cheesecloth Beakers Dropper Blender PROCEDURE: 1. Cut potato. Blend 15g in 100ml distilled water. 2. Filter through cheesecloth. 3. Collect filtrate. Divide into 2 lots. 4. Boil 1 Lot for 3 minutes (Lot A). Leave the other Lot unheated (Lot B). 5. Fill each of three test tubes % full (3ml) of distilled water. 6. Add 10 drops of catechol to each tube. 7. Add 10 drops of heated potato extract (Lot A) to 1 test tube. Mix. 8. Add 10 drops of unheated potato extract (Lot B) to another test tube. Mix. 9. Add 10 drops of distilled water (Control) to the third test tube. Mix. 10. Note and record color of each tube at 1-minute intervals for 5 to 10 minutes.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In this experiment of polyphenoloxidase, why does the longer the time, the darker the color is ithe result of the unheated sample?

MATERIALS:
Potato Thermometer
Test Tubes Bunsen burner and tripod set up Distilled water 0.1%
Catechol solution (in amber bottle) Cheesecloth Beakers
Dropper Blender
PROCEDURE:
1. Cut potato. Blend 15g in 100ml distilled water.
2. Filter through cheesecloth.
3. Collect filtrate. Divide into 2 lots.
4. Boil 1 Lot for 3 minutes (Lot A). Leave the other Lot unheated (Lot B).
5. Fill each of three test tubes % full (3ml) of distilled water.
6. Add 10 drops of catechol to each tube.
7. Add 10 drops of heated potato extract (Lot A) to 1 test tube. Mix. 8. Add 10
drops of unheated potato extract (Lot B) to another test tube. Mix. 9. Add 10
drops of distilled water (Control) to the third test tube. Mix. 10. Note and record
color of each tube at 1-minute intervals for 5 to 10 minutes.
Transcribed Image Text:MATERIALS: Potato Thermometer Test Tubes Bunsen burner and tripod set up Distilled water 0.1% Catechol solution (in amber bottle) Cheesecloth Beakers Dropper Blender PROCEDURE: 1. Cut potato. Blend 15g in 100ml distilled water. 2. Filter through cheesecloth. 3. Collect filtrate. Divide into 2 lots. 4. Boil 1 Lot for 3 minutes (Lot A). Leave the other Lot unheated (Lot B). 5. Fill each of three test tubes % full (3ml) of distilled water. 6. Add 10 drops of catechol to each tube. 7. Add 10 drops of heated potato extract (Lot A) to 1 test tube. Mix. 8. Add 10 drops of unheated potato extract (Lot B) to another test tube. Mix. 9. Add 10 drops of distilled water (Control) to the third test tube. Mix. 10. Note and record color of each tube at 1-minute intervals for 5 to 10 minutes.
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