TEST ONE: When one uses Benedict’s reagent added to UNKNOWN samples taken and added (as a liquid) by a pipette (in our homes we call them eye-droppers) from each container into a labeled by number test tube. The seven labeled test tubes are then immersed into a beaker on a hot plate with warm water in it (called a water bath): Samples ONE TWO THREE FOUR FIVE SIX SEVEN All seven UNKNOWNS having Benedicts reagent added to them then change from transparent clear after to the same color as the Benedict’s reagent, when the blue colored reagent is added to them. They are all (all seven unknowns) now blue in color. Then the seven test tubes are immersed in a warm water bath. What is seen by you is that NO color change occurs initially when they are all immersed in warm water. THEN, over a five to ten-minute window of time as you watch the unknown test tube containing samples in the warm water bath, the test tubes containing unknowns ONE and FOUR change in color, to your eyes, to an identified a positive color change of ORANGE/RED/white. TEST TWO: When one uses the Biuret reagent on samples from test tubes TWO, and THREE, a Pink color forms, and in sample FIVE the chemical unknown changes to a Violet purple color. IN UNKNOWNS ONE, FOUR, SIX, and SEVEN these unknowns all stay the blue color of the Biuret reagent that was added to the initially clear samples. NO color change occurs in these. TEST THREE: When one adds Sudan IV to all seven samples ONLY sample SIX reacts and the liquid of the unknown sample becomes a reddish / burgundy color. Samples ONE, TWO, THREE, FOUR, FIVE, and SEVEN turn color to a dilute red color from the stain added to the clear samples, but not in the intensity that is sample unknown SIX. What can you deduce for the preceding set of experiments as to what was in each sample regarding? Monosaccharide / reducing sugar, a short chain polypeptide (a short protein), a long chain polypeptide (a long protein), or a lipid (a wax or an oil). Using the data now that you have by this “experiment”, identify here, in the spaces provided (or on a sheet of paper) what each UNKNOWN now by you is known to be. Sample Unknown FOUR is: _____________________ Sample Unknown FIVE is: _____________________ Sample Unknown SIX is: _____________________ Sample Unknown SEVEN is: _____________________