Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include chemical reactions if necessary.
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Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include
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- Complete the table below by adding (+) or (-) if eahc sample (amino acid or protein) below will test positive or negative, respectively. sample BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOSKIN-COLE G W H Y C R HAIR GELATIN CASEIN PEPTONE Identify the amino acid that will give the following data: BIURET NINHYDRIN XANTHOPROTEIC MILLON-NASSE SAKAGUCHI LEAD ACETATE PAULY HOPSKIN-COLE - + + + - - + - Name of amino acid: structurte of the amino acid:A plant isolate was subjected to qualitative tests to determine the presence of macromolecules. Below is a summary of results obtained: Biuret Ninhydrin Schiff's Test for Test for Deoxyribose Phosphat test test test Violet Violet Pink Blue Yellow solution solution solution solution precipitat To form a definitive conclusion about the isolate, what additional test is needed (if any)? O Wheeler-Johnson Test Sakaguchi Test Molisch Test Dische Diphenylamine TestA mixture of lipids containing phosphatidic acid, cholesterol, testosterone, phosphatidylserine, andphosphatidylethanolamine was applied to a hydrophobic interaction chromatography column. Thecolumn was washed with a high salt buffer, and the lipids were then eluted with decreasing saltconcentrations. In what order would the lipids be eluted from the column? Explain your answer.
- A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingDesign an assay with purified phycocyanin to test protein stability using 500ml of 95% ethanol. Then do the same with 2 M sodium chloride. Make each step clear.Give the chemical principles involved in a positive Biuret test, Millon’s Test and Sakaguchi Test. What is the chemical basis of the Ninhydrin Test, Hopkins – Cole test and Sulfur reaction test? What chemical structure should be present in an amino acid or protein to give a positive Xanthoproteic test?
- What types of macromolecules are usually separated on agarose electrophoresis gels?Give other chromatographic techniques that can be used for separating non-polar biomolecules, such as lipids. Explain the principle in terms of lipid separation.In a mixture of five proteins listed, draw an elution profile (Absorbance vs. mL eluted, arbitrary) for the purification of the listed proteins on a gel filtration chromatography resin: cytochrome c (pI = 5.4; Mr = 13,000), immunoglobulin G (pI = 7.3; Mr = 145,000), ribonuclease A (pI = 9.6; Mr = 13,700), RNA polymerase (pI = 6.3; Mr = 450,000), human serum albumin (pI = 5.4; Mr = 68,500). Label your elution peaks. Draw a sketch of an SDS-PAGE, reflecting the mobility of the above mixture as they elute from the column. Label you protein bands.
- Multiple choice: (use the picture as reference) Will this be soluble to CHCl3? * yes no Which part of the structure will react to Liebermann-Burchard test? * C17 -OH group Ring A Rings C and D Which of the statements is NOT TRUE about the sample in Salkowski test? Lower layer becomes red with a green fluorescence A molecule of cholesterol reacts with sulfuric acid to yield bi-cholestadien There should be an excess sulfuric acid to convert bi-cholestadien to bisulfonic acid of bi-cholestadien. It is best to shake the solution for a more accurate result.Provide examples of amino acids that would yield a positive Azo Dye test and amino acids that would yield a negative Azo Dye test. Show the structures.Arrange the following in order of increasing Rf on thin-layer chromatography: acetic acid, acetaldehyde, 2-octanone, decane, and 1-butanol.