Activity of phosphofructokinase-1 (PFK-1) is tightly-controlled by allosteric regulators. Identify the two positive and two negative allosteric regulators of PFK-1 activity from the list of biomolecules below. In addition, identify the two biomolecules in the list that do not function as allosteric regulators of PFK-1. Type of PFK-1 Regulation A. Positive allosteric regulation Biomolecules 1. fructose- 1,6-BP B. Negative allosteric 2. citrate regulation C. Not an allosteric 3. ATP regulator 4. ADP 5. glucose- 6P 6. fructose-

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**Activity of Phosphofructokinase-1 (PFK-1) and Allosteric Regulation**

Phosphofructokinase-1 (PFK-1) plays a critical role in glycolysis and is tightly controlled by allosteric regulators. The task is to identify two positive and two negative allosteric regulators of PFK-1 activity from the list of biomolecules provided. Additionally, identify two biomolecules in the list that do not function as allosteric regulators of PFK-1.

**Types of PFK-1 Regulation**

**A. Positive Allosteric Regulation**
- These are molecules that enhance the activity of PFK-1.

**B. Negative Allosteric Regulation**
- These molecules inhibit the activity of PFK-1.

**C. Not an Allosteric Regulator**
- These molecules do not affect PFK-1 regulation.

**Biomolecules**
1. Fructose-1,6-bisphosphate (fructose-1,6-BP)
2. Citrate
3. ATP
4. ADP
5. Glucose-6-phosphate (glucose-6P)
6. Fructose-2,6-bisphosphate (fructose-2,6-BP)
Transcribed Image Text:**Activity of Phosphofructokinase-1 (PFK-1) and Allosteric Regulation** Phosphofructokinase-1 (PFK-1) plays a critical role in glycolysis and is tightly controlled by allosteric regulators. The task is to identify two positive and two negative allosteric regulators of PFK-1 activity from the list of biomolecules provided. Additionally, identify two biomolecules in the list that do not function as allosteric regulators of PFK-1. **Types of PFK-1 Regulation** **A. Positive Allosteric Regulation** - These are molecules that enhance the activity of PFK-1. **B. Negative Allosteric Regulation** - These molecules inhibit the activity of PFK-1. **C. Not an Allosteric Regulator** - These molecules do not affect PFK-1 regulation. **Biomolecules** 1. Fructose-1,6-bisphosphate (fructose-1,6-BP) 2. Citrate 3. ATP 4. ADP 5. Glucose-6-phosphate (glucose-6P) 6. Fructose-2,6-bisphosphate (fructose-2,6-BP)
This image contains a matching exercise related to allosteric regulation, a concept in biochemistry. It is likely intended for educational purposes.

### Categories and Options:

**A. Positive allosteric regulation**  
**B. Negative allosteric regulation**  
**C. Not an allosteric regulator**  

### Options:  
1. citrate  
2. ATP  
3. fructose-2,6-BP  
4. AMP  
5. glucose-6P  
6. fructose-2,6-BP  

### Answer Selections:

- **Option 1:** \(A: 4,6; \ B: 1,3; \ C: 2,5\)
- **Option 2:** \(A: 3,6; \ B: 2,4; \ C: 1,5\)
- **Option 3:** \(A: 4,6; \ B: 2,3; \ C: 1,5\)
- **Option 4:** \(A: 4,5; \ B: 2,3; \ C: 1,6\)
- **Option 5:** \(A: 3,5; \ B: 1,4; \ C: 2,6\)

This exercise requires students to match metabolites with their role in allosteric regulation based on the provided categories. Each option presents a different combination for categorizing the substances.
Transcribed Image Text:This image contains a matching exercise related to allosteric regulation, a concept in biochemistry. It is likely intended for educational purposes. ### Categories and Options: **A. Positive allosteric regulation** **B. Negative allosteric regulation** **C. Not an allosteric regulator** ### Options: 1. citrate 2. ATP 3. fructose-2,6-BP 4. AMP 5. glucose-6P 6. fructose-2,6-BP ### Answer Selections: - **Option 1:** \(A: 4,6; \ B: 1,3; \ C: 2,5\) - **Option 2:** \(A: 3,6; \ B: 2,4; \ C: 1,5\) - **Option 3:** \(A: 4,6; \ B: 2,3; \ C: 1,5\) - **Option 4:** \(A: 4,5; \ B: 2,3; \ C: 1,6\) - **Option 5:** \(A: 3,5; \ B: 1,4; \ C: 2,6\) This exercise requires students to match metabolites with their role in allosteric regulation based on the provided categories. Each option presents a different combination for categorizing the substances.
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