-Absorption maximum at 323 nm at time = 0 minutes (absorption = 0.0625). Use this absorption to calculate the molar absorptivity; the initial concentration = 1.00 x 10-5 M -Use the following kinetic data to determine the time-integrated rate law. This can be done by using Excel to plot In [ ] vs. time and 1/[ ] vs. time and determine which plot has a better linear fit. Absorbance 0.044705 time 60 min 0.041725 120 min 0.039537 180 min 0.036528 240 min 0.034014 300 min Compound 2-[(di-sec-butyl-methyl-phenanthroline)AuC13] (di-sec-butyl-methyl-phenanthroline = 2,9- di-sec-butyl-4-methyl-1,10-phenanthroline) Cl CI Au' N CI

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5. The table shows intensities and lifetime data for luminescence of a ruthenium complex for a range of oxygen concentrations. Concentration Fluorescence Fluorescence intensity oxygen (uM) lifetime (us) 590 1.008 22 574 0.978 44 557 0.964 72 552 0.95 132 518 0.916 174 503 0.893 267 478 0.836 a. Why does the luminescence intensity decrease as the oxygen concentration increases? The luminescence intensity decreases as the oxygen concentration increases because the oxygen is acting as a quencher so when the oxygen is present the luminescence gets excited and then that energy is transferred and accepted by охудen. b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear? The calibration is c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?

The observed fluorescence lifetime in the absenceof a quencher is 1.4 ns. In the presence of a quencher thefluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the fluorescence quantum yieldsin the presence and absence of the quencher. ¢,/¢,_0

Crystal violet is a brightly colored, purple dye that can be used as a pH indicator. In the presence of a base, crystal violet reacts with OH- to become a colorless molecule. Since crystal violet is colorful violet (i.e. purple) molecule, the kinetics of this reaction can be investigated using absorbance spectroscopy as the violet color disappears. What color of light should be used for measuring the absorbance over time?

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What is the order of a reaction if the slope of a line for experimental data is negative and the rate of the reaction was found to be independent of the absorbance?

Mulecular Absorption Spectrometry MacBook Pro 6. Describe the advantage of a four-level laser system over a three-level type. 7. The reduced form of nicotinamide adenine dinucleotide (NADH) is an important and highly fluorescent coenzyme. It has an absorption maximum of 340 nm and an emission maximum at 465 nm. Standard solutions of NADH gave the following fluorescence intensities: Relative Intensity Conc. NADH, umol/L 0.100 2.24 0.200 4.52 0.300 6.63 9.01 0.400 0.500 10.94 0.600 13.71 0.700 15.49 0.800 17.91 A) Construct a spreadsheet and use it to draw a calibration curve for NADH. B) Find the least-squares slope and intercept for the plot in (A) C) An unknown exhibit a relative fluorescence intensity of 12.16. Use the spreadsheet to calculate the concentration of NADH. 8. The IR spectrum of CO shows a vibrational absorption band centered at 2170/cm. (a) what is the force constant for the CO bond? (b) At what wavenumber would the corresponding peak for "CO occur? 9. Calculate the…

In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming that 7500 counts represents a FRET efficiency of 0.6, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine-texas red FRET pair employed ro = 50. Å. Express your answer to two significant figures and include the appropriate units. HÀ ? Ar = Value Units

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