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5. The table shows intensities and lifetime data for luminescence of a ruthenium complex for a range of oxygen concentrations. Concentration Fluorescence Fluorescence intensity oxygen (uM) lifetime (us) 590 1.008 22 574 0.978 44 557 0.964 72 552 0.95 132 518 0.916 174 503 0.893 267 478 0.836 a. Why does the luminescence intensity decrease as the oxygen concentration increases? The luminescence intensity decreases as the oxygen concentration increases because the oxygen is acting as a quencher so when the oxygen is present the luminescence gets excited and then that energy is transferred and accepted by охудen. b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear? The calibration is c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?

5. The table shows intensities and lifetime data for luminescence of a ruthenium complex for a range of oxygen concentrations. Concentration Fluorescence Fluorescence intensity oxygen (uM) lifetime (us) 590 1.008 22 574 0.978 44 557 0.964 72 552 0.95 132 518 0.916 174 503 0.893 267 478 0.836 a. Why does the luminescence intensity decrease as the oxygen concentration increases? The luminescence intensity decreases as the oxygen concentration increases because the oxygen is acting as a quencher so when the oxygen is present the luminescence gets excited and then that energy is transferred and accepted by охудen. b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear? The calibration is c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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5. The table shows intensities and lifetime data for luminescence of a ruthenium complex for a range of oxygen concentrations. Concentration Fluorescence Fluorescence intensity oxygen (uM) lifetime (us) 590 1.008 22 574 0.978 44 557 0.964 72 552 0.95 132 518 0.916 174 503 0.893 267 478 0.836 a. Why does the luminescence intensity decrease as the oxygen concentration increases? The luminescence intensity decreases as the oxygen concentration increases because the oxygen is acting as a quencher so when the oxygen is present the luminescence gets excited and then that energy is transferred and accepted by охудen. b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear? The calibration is c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?
Transcribed Image Text:5. The table shows intensities and lifetime data for luminescence of a ruthenium complex for a range of oxygen concentrations. Concentration Fluorescence Fluorescence intensity oxygen (uM) lifetime (us) 590 1.008 22 574 0.978 44 557 0.964 72 552 0.95 132 518 0.916 174 503 0.893 267 478 0.836 a. Why does the luminescence intensity decrease as the oxygen concentration increases? The luminescence intensity decreases as the oxygen concentration increases because the oxygen is acting as a quencher so when the oxygen is present the luminescence gets excited and then that energy is transferred and accepted by охудen. b. Plot a Stern-Volmer graph based on fluorescence intensity. Is the calibration linear? The calibration is c. Plot a Stern-Volmer graph based on fluorescence lifetime. What type of quenching is indicated by the plot?
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