The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching F(M) 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70,14 0.015 54.36 0.020 44.14 0.025 39.18 0.030 30.25 0.040 Fluorescen 24.27 0.050 20.95 0.060 Ksy= M
The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching F(M) 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70,14 0.015 54.36 0.020 44.14 0.025 39.18 0.030 30.25 0.040 Fluorescen 24.27 0.050 20.95 0.060 Ksy= M
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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![The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F)
intensities for fluorescein as a function of the iodide ion concentration in solution are
shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching
] (M)
F
272.6
0.000
constant, Ksy.
135.3
0.0050
HO
89.12
0.010
70.14
0.015
54.36
0.020
44.14
0.025
.COOH
39.18
0.030
30.25
0.040
Fluorescein
24.27
0.050
20.95
0.060
Ksy =](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0b0f4c6a-3966-4afa-a943-fc4d75b024e6%2Fc3b67fce-dd56-4e25-8843-c0dd825859c5%2Foio1rra_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F)
intensities for fluorescein as a function of the iodide ion concentration in solution are
shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching
] (M)
F
272.6
0.000
constant, Ksy.
135.3
0.0050
HO
89.12
0.010
70.14
0.015
54.36
0.020
44.14
0.025
.COOH
39.18
0.030
30.25
0.040
Fluorescein
24.27
0.050
20.95
0.060
Ksy =
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