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The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching F(M) 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70,14 0.015 54.36 0.020 44.14 0.025 39.18 0.030 30.25 0.040 Fluorescen 24.27 0.050 20.95 0.060 Ksy= M

The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching F(M) 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70,14 0.015 54.36 0.020 44.14 0.025 39.18 0.030 30.25 0.040 Fluorescen 24.27 0.050 20.95 0.060 Ksy= M

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching ] (M) F 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70.14 0.015 54.36 0.020 44.14 0.025 .COOH 39.18 0.030 30.25 0.040 Fluorescein 24.27 0.050 20.95 0.060 Ksy =
Transcribed Image Text:The fluorescence of fluorescein is quenched by the iodide (I") ion. The fluorescence (F) intensities for fluorescein as a function of the iodide ion concentration in solution are shown in the table. Prepare a Stern-Volmer plot to determine the Stern-Volmer quenching ] (M) F 272.6 0.000 constant, Ksy. 135.3 0.0050 HO 89.12 0.010 70.14 0.015 54.36 0.020 44.14 0.025 .COOH 39.18 0.030 30.25 0.040 Fluorescein 24.27 0.050 20.95 0.060 Ksy =
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