A. Use the following table to answer the carbohydrate-related questions (1 - 4): Tube Solution Reaccion of color Benedict Reaccion of color mud 1 10 drops of onion juice blue Ambar 2 10 drops of potato juice blue Ambar 3 10 drops of sacarosa solution Red yellow 4 10 drops de solución de glucosa orange yellow 5 10 drops of destilled water blue yellow 6 10 drops of sugar reductor solution green yellow 7 10 drops of almidon solution blue Ambar 1. In Benedict's test, which of the solutions is a negative control? Positive? 2. What is a reducing sugar, glucose or sucrose? Explain. 3. Which contains more reducing sugars, potato juice or onion juice? Explain.
A. Use the following table to answer the carbohydrate-related questions (1 - 4):
|
Tube |
Solution |
Reaccion of color Benedict |
Reaccion of color mud |
|
1 |
10 drops of onion juice |
blue |
Ambar |
|
2 |
10 drops of potato juice |
blue |
Ambar |
|
3 |
10 drops of sacarosa solution |
Red |
yellow |
|
4 |
10 drops de solución de glucosa |
orange |
yellow |
|
5 |
10 drops of destilled water |
blue |
yellow |
|
6 |
10 drops of sugar reductor solution |
green |
yellow |
|
7 |
10 drops of almidon solution |
blue |
Ambar |
1. In Benedict's test, which of the solutions is a negative control? Positive?
2. What is a reducing sugar, glucose or sucrose? Explain.
3. Which contains more reducing sugars, potato juice or onion juice? Explain.
4. In the starch iodine test, which solution is a negative control? Positive?
B. Use the following table to answer the questions related to the Biuret protein test (5 - 7):
|
Tube |
Solution |
Reaccion of color |
|
1 |
2 mL egg albumin |
Violet |
|
2 |
2 mL honey |
blue |
|
3 |
2 mL solution of amino acid |
Violet |
|
4 |
2 mL destilled water |
blue |
|
5 |
2 mL solution of protein |
Violet |
5. In the Biuret test, which of the solutions is a negative control? Positive?
6. Which contains more protein (C-N bonds): egg albumin or honey? Explain.
7. Do free amino acids have peptide bonds?
C. Answer the following questions:
8. Why does the methylene blue dye stain the nuclei in onion cells?
9. What is the cause of the variation in color intensity in a biochemical test?
10. You have negative results on both the Benedict and Biuret tests but both look blue. Why?
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