A type of phosphatase can be assayed using p-nitrophenyl phosphate. The assay is performed at pH 7.8, which is the optimum pH for this enzyme. The initial velocity measured at the highest substrate concentration was 0.025 ΔA400nm/minute. Convert this velocity to a change in concentration/minute (μM/min). Use 18,380M^-1cm^-1 for the extinction coefficient.

Q: The Km of an enzyme is 5.0 mM. Calculate the substrate concentration when this enzyme operates at…

A: Given data :

Q: Consider the Michaelis-Menten equation and the definition of Km under steady state conditions. Under…

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Q: In the "Catalysis by Preferential Transition State Binding" mechanism, the substrate perfectly fits…

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Q: The equilibrium constant for the attachment of a substrate to the active site of an enzyme was…

A: In the reaction considered, the loss of unreacted substrate is basically the backward reaction with…

Q: What is the Vmax of an enzyme at 1 µM with an activation free energy (AG#) of 55000 J/mol at 298K?…

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Q: For an enzyme present at a concentration of 5 µM, the following initial velocities have been…

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Q: 4) In the lab you are extracting and enzyme called catalase from a potato and want to use this…

A: Enzymes are commonly help in speed up the reaction. Catalase is an enzyme that is found in almost…

Q: Calculate the substrate concentration of an enzyme catalyzed reaction whose rate is equal to half of…

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Q: 2. Using the following data, determine the Vmax and Km of the enzyme catalyzed reaction: [S] (M) 2.5…

A: The objective of this question is to determine the maximum velocity (Vmax) and the Michaelis…

Q: The figure below shows the dependence of the enzyme's rate, v (in uM/min), as a function of…

A: Lineweaver–Burk plot gives the value of Vmax for the enzyme.

Q: The equilibrium constant for the binding of a substrate to the active site of an enzyme was measured…

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Q: Enzyme kinetics a. How to determine Km and Vmax of a-glucosidase enzyme isolated from weathered rice

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Q: What is the turnover number in molecules/min of a catalase enzyme molecule that cleaves 8.4 x 107…

A: Given the number of molecules cleaved = 8.4*107 H2O2 molecules time = 15 min

Q: The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial…

A: The above question is based on enzyme catalysis. We have to discuss the given conditions on the rate…

Q: The following results were obtained for an enzyme-catalyzed reaction Substrate concentration (mmol…

A: The objective of this question is to calculate the Michaelis-Menten constant (Km) and the maximum…

Q: ES → ES' v = k,[ES] ES' → E+ P v = k,[ES'] Show that the rate of formation of product has the same…

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Q: An enzyme catalyzes a reaction with a KmKm of 7.507.50 mM and a VmaxVmax of 3.00 mM⋅s−1.3.00 mM⋅s−1.…

A: Given: Km = 7.50 mM  Vmax = 3.00 mM⋅s−1

Q: The following data were obtained in a study of an enzyme known to follow Michaelis-Menten kinetics.…

A: The value of km can be calculated by plotting the graph according to the Michelis -Menten equation.

Q: Reversible Enzyme Kinetics: Consider the following reversible reaction: k, k. E+S ES P+E k_1 Show…

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Q: You performed a kinetic study of the enzyme lipase and you obtained the following double reciprocal…

A: This is based on enzyme catalysis . We have to select true statement from the above given points.…

Q: CH Vacant d-orbital of metal

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Q: At what substrate concentration would an enzyme with a kcat of 30.0 s-1 and a Km of 3. (а) 0.0050 M…

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Q: Suppose that you have an enzyme concentration at the molarity calculated in question 2 and the…

A: Detailed explanation:Let's break down the calculation of kcat (catalytic efficiency) and KM…

Q: Assume that an enzyme has the following kinetic constants: Vmax = 50 umol substrate transformed per…

A: Michaeli's‐Menten's equationrate of r×n:V0=VmaxSKm+Swhere,  Vmax=enzyme's maximum rate.…

Q: Kinetics data were gathered for an enzyme-catalyzed reaction under identical conditions both with…

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Q: The Michaelis-Menten equation is often used to describe the kinetic characteristics of an…

A: According to the Michaelis-Menten equation the rate of an enzyme-catalyzed reaction is given by:…

Q: Q7. Kinetics were analysed for an enzyme in the absence and presence of inhibitor. In the absence of…

A: A multiple choice question based on kinetics of reactions that is to be accomplished.

Q: The substrate dissociation constant, Ks, is k.1/k1. What do large values of this constant mean? That…

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Q: Determine the values of KM and Vmax. For the Vmax obtained, calculate the turnover number assuming…

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Q: Determine the values of KM and Vmax. For the Vmax obtained, calculate the turnover number assuming…

A: The graph formed will be as follows: According to the Michaelis Menten equation where  R is the…

Q: protein catalyzes the following reaction and had a Michaelis-Menten constant of  Km=25 mM and a…

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Q: Relation between Reaction Velocity and Substrate Concentration: MichaelisMenten Equation: At what…

A: Write the expression of Michaelis Menten Equation. Vo = Vmax×[S]KM+ [S]  where Vo is the velocity or…

Q: Given the following data for an enzyme-catalyzed reaction. Substrate conc., mM Velocity, mM/s 0.10…

A: Lineweaver Burk equation is the plot of 1/Velocity as y as a function of 1/concentration. The…

Q: 5. Varying the enzyme- For a one substrate, enzyme-catalyzed reaction, double reciprocal plots were…

A: The Vmax (maximum rate or velocity) directly related to the concentration of the enzyme, [E], means…