A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm-² on acuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibrationcurve of the same molecule is given. Predict quantum yield.1.00E+01Flurorescence (A.U.)1.00E+001.00E-011.00E-021.00E-031.00E-040.001O 0.1 absorbance units4x10 fluorescence unitsy = 0.1969x + 0.0019R² = 1O 8x10+ fluorescence units0.01Cannot be determined with the current information.0.1Concentration (mm)110

A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm² on a cuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibration curve of the same molecule is given. Predict quantum yield.

A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm² on a cuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibration curve of the same molecule is given. Predict quantum yield.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm-² on a
cuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibration
curve of the same molecule is given. Predict quantum yield.
1.00E+01
Flurorescence (A.U.)
1.00E+00
1.00E-01
1.00E-02
1.00E-03
1.00E-04
0.001
O 0.1 absorbance units
4x10 fluorescence units
y = 0.1969x + 0.0019
R² = 1
O 8x10+ fluorescence units
0.01
Cannot be determined with the current information.
0.1
Concentration (mm)
1
10

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