A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm-² on acuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibrationcurve of the same molecule is given. Predict quantum yield.1.00E+01Flurorescence (A.U.)1.00E+001.00E-011.00E-021.00E-031.00E-040.001O 0.1 absorbance units4x10 fluorescence unitsy = 0.1969x + 0.0019R² = 1O 8x10+ fluorescence units0.01Cannot be determined with the current information.0.1Concentration (mm)110

A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm² on a cuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibration curve of the same molecule is given. Predict quantum yield.

A sample that is 10 mM in concentration with a molar absorbance coefficient of 20 M ¹cm² on a cuvette that is 0.5 cm in path length is analyzed by a laser for its fluorescence behavior. A calibration curve of the same molecule is given. Predict quantum yield.

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis

Section: Chapter Questions

Problem 4Q

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Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of solution.A 10.00-mL aliquot was then diluted to 50.00 mL with the acid. The fluorescence intensity for the dilutedsample at 347.5 nm provided a reading of 196 on an arbitrary scale. A standard 100-ppm quinine solutionregistered 125 when measured under conditions identical to those for the diluted sample. Calculate the massin milligrams of quinine in the tablet.
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