A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas 2.3, 9.7) at pH 7 is loaded onto an SP-Sepharose resin and then eluted at pH 7 using a gradient of increasing NaCl concentration. What would be the order of elution of the amino acids from the column (first to last)?

A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas 2.3, 9.7) at pH 7 is loaded onto an SP-Sepharose resin and then eluted at pH 7 using a gradient of increasing NaCl concentration. What would be the order of elution of the amino acids from the column (first to last)?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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