Finally, you want to make completely certain that you purified the correct protein and not some other protein (like biochemisnotfunase). To confirm the identity of your protein, you run a Western blot. When doing a Western, one typically needs a solution of phosphate-buffered saline, which is comprised of 10 mM phosphate buffer (pH 8.0) and 0.9% (w/v) NaCl. Describe how you would make 500 mL of this solution including the grams of sodium phosphate, monobasic and sodium phosphate, dibasic that you would need.
Coding Strand of DNA
When pointing to DNA transcription, the coding strand is found to be the DNA strand whose base sequence is indistinguishable from the base sequence of the RNA transcript developed. It is this strand that comprises the codons, while the non-coding strand comprises the anti-codons.
Nucleotide
Both DNA and RNA are composed of organic molecules known as nucleotides. Hence, nucleotides are known as the basic building blocks of nucleic acids. These substances play a role in various processes such as cell signalling, enzyme reactions, metabolism, and so on.
Structure of Cytosine
Cytosine is among the five primary nitrogenous bases of which DNA and RNA and are being used in storage and transportation of genetic makeup within a cell. Adenine, guanine, thymine as well as uracil are the remaining four nucleobases.
Finally, you want to make completely certain that you purified the correct protein and not some other protein (like biochemisnotfunase). To confirm the identity of your protein, you run a Western blot. When doing a Western, one typically needs a solution of phosphate-buffered saline, which is comprised of 10 mM phosphate buffer
(pH 8.0) and 0.9% (w/v) NaCl. Describe how you would make 500 mL of this solution including the grams of sodium phosphate, monobasic and sodium phosphate, dibasic that you would need.
Making the phosphate buffer is a little tricky. Be sure to use the correct ratio of monobasic and dibasic sodium phosphate to get the correct pH. The Henderson- Hasselbalch equation might come in handy here. Finally, the molecular weight of sodium phosphate, monobasic (NaH2PO4) is 119.98 g/mol and the molecular weight of sodium phosphate, dibasic (Na2HPO4) is 141.96 g/mol.
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