A researcher is preparing a reaction mixture to test the activity of a protein.They combine at room temperature the required reaction components, which contained in a final 150 ml volume 200 mMNaCl and unknown concentrations of weak acid and conjugate base of a buffer compound. They are using a pH meter todetermine the acidity of the solution and observe the following pH value that is displayed by the device.Can you determine the hydrogen ion [H+] concentrationof the solution?Provide the value with three decimal places andan appropriate unit in the answer box.Note: You may need to round the numbers to get the required answer.N3.35

pH is the negative log of H+ concentration, where the H+ concentration is in molar (M) pH = -log[H+]…

Answered: A researcher is preparing a reaction…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.
explain step by step why the correct answer is C
1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:
How do I calculate the final concentration? I just need one solution to walk through the steps. Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL 0.00 mL 10.00 mL 2.50 mL 1.00 mL 0.50 mL 10.00 mL 2.50 mL 1.00 mL 1.00 mL 10.00 mL 2.50 mL 1.00 mL 2.00 mL 10.00 mL 2.50 mL 1.00 mL 3.00 mL 10.00 mL 2.50 mL 1.00 mL 4.00 mL 10.00 mL 2.50 mL 1.00 mL 5.00 mL 10.00 mL 2.50 mL 1.00 mL 6.00 mL 10.00 mL
In protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8
A purified protein fraction has a total sample volume of 450 µL. The sample has a corrected A280 of 0.244, and the blank corrected A280 was 0.077. (Both values were measured with a path length of 1.00 cm.) If 5.50 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
An enzyme uses a lysine and a histidine as general acid-base catalytic residues. In one direction of the reaction, the lysine and histidine residues are typically protonated (~NH3*, ~Im), but in the other direction of the reaction, the two residues are "reverse protonated" (~NH2, ~Hlm*) (see below scheme). A pH profile of the reaction at various pH values yielded estimates of 7.57 and 9.30 for the macroscopic ionization constants pk,' and pK2, respectively. A separate set of experiments with site-speciffic variants of the enzyme yielded an estimate of 8.78 for the microscopic ionization constant pKp. Given this information, what is the value of the microscopic ionization constant pKA? Report your answer to the nearest hundredth. Lys-NH3* His-HIm" KB H KA H* Lys-NH2 Lys-NH3* His-HIm" His-Im Kc H* Kp Lys-NH2 His-Im
A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
To prepare a gel sample, you want to load 50 ng total of protein/well. You have added 200 μL of protein in 800 µL reagent and measured your sample by Bradford A595 to be 0.7 mg/mL - your dilution is unaccounted for at this point. Assuming a total final volume of 20 μL, what volume of protein sample, buffer and 4X SDS PAGE Loading Dye needs to be mixed to create a final sample of 50 ng protein in 20 μL?

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS