A particular food coloring has a molarabsorptivity of 3.8*10 3 cm -1 M -1 at 510nm. What will be the absorbance of a 2*10-4M solution in 1 -cm cuvette at 510 nm? What will be the percent transmittance of thesolution in (a)?

Q: A solution of a specific vitamin has a åmax of 271 nm and a concentration of 2.73 × 10-7M. The…

A: Given that : Concentration of the solution (C) = 2.73 x 10-7 M Absorbance of the solution (A) =…

Q: extinction coefficient = ε, path length = b, concentration C A sample containing an unknown…

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Q: What is the

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Q: Ni2+ appears green in solution, so its concentration can be determined spectrophotometrically.  A…

A: Since in absorbance v/s concentration plot, we plot the absorbance value in y axis and concentration…

Q: If the measured absorbance at 280 nm of the protein in a 1.00-cm cuvette = 0.564, what is the…

A: Solution -  Beer-Lambert law state   The Beer-Lambert law states that there is a linear…

Q: 3. A 2.50×104M solution of a dye has an absorbance of 0.680 when measured in a 1.00 cm cell at 625…

A: The relation between absorbance and transmittance is given by A=2-logT     or A=-logT where A=…

Q: A blue product absorbs light at 590 nm (ε590nm = 7,600 M-1 cm-1). Its concentration was too…

A: The actual concentration is 0.459 mM.

Q: A student prepared several solutions of an unknown molecule, each at a different concentration. The…

A: Given: Best fit line y= 0.00626x + 0.00732 Path length= 0.760 cm To find: molar absorptivity

Q: A 2.54 x 10- M sample of compound X in a solvent has an absorbance of 0.370 at 528 nm in a 1.000 cm…

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Q: A 1.3 * 10-5M solution of an unknown substance has an absorbance of 0.345 at its λmax. Calculate the…

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Q: A solution of a specific vitamin has a Âmax of 245 nm and a concentration of 4.15 × 10-/M. The…

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Q: Calculate the absorbance, A, and percent transmittance, T, of a 0.00245 M solution that has a molar…

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Q: A student prepared several solutions of Molecule Bright, each at a different concentration. The…

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Q: A 50 ml sample of Ni2+ gave an absorbance of 0.455 in an atomic absorption analysis. Then 2.00 ml of…

A: Use the following equation. is the unknown initial concentration of analyte in the sample is the…

Q: What absorbance value corresponds to 41.0% T'? A = A 0.0148 M solution of a certain analyte exhibits…

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Q: A standard 3 mL cuvette is two-thirds filled with a 1 in 5 dilution of 70 mM oxyhaemoglobin. The…

A: In the presence of a live yeast suspension, the absorbance is measured to be 0.4, which is 0.2 units…

Q: Please refer to the graph in the lab document. If the absorbance of a solution containing FeSCN2+ is…

A: Given,                           Concentration of [FeSCN]2+      Absorbance             Percent…

Q: At 590 nm, crystal violet (a biological stain) has a molar absorptivity (ε) of 8.7×104 M-1cm-1.…

A: Spectrophotometry is a method to measure how much a substance absorbs light by measuring the…

Q: Based on the calibration curve, what is the molar concentration for a solution with a measured…

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Q: Absorbance 0.350 0.300 0.250 0.200 0.150 0.100 0.050 Chart Title +Series 1

A: Given :- % transmittance reading of dye = 61.6% Concentration of dye = ?

Q: The molar absorptivity of a colored solution with a concentration of 0.0222M was determined to be…

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Q: A 0.012mM sample of a compound in water has a molar absorptivity of 27,2671 1/m^-1cm^-1 at 573nm in…

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Q: A student creates a sports drink by dissolving 5.0 g of powder in 250 mL of water. The green colour…

A: Given, absorbance= 0.450  Molecular weight of dye X is 418.4 g/mol  Mass of powder is 5 g  We know…

Q: The validation process comprises four main steps that are : hardware validation, software…

A: Validation in analytical chemistry is the process of verifying that a procedure yield acceptable…

Q: A specific steroid has a Amax of 251 nm and a molar absorptivity e of 11, 500 L mol-¹ cm-¹. What is…

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Q: A solution prepared by dissolving 25.8 mg of benzene (78.11 g/mol) in hexane (86.16 g/mol) and…

A: Here we have to just calculate the concentration. We don't require the absorption value. The…

Q: The ultraviolet spectrum of aspirin shows a secondary absorption band at 271 nm. If a solution of…

A: The absorbing species obey Lambert beer law

Q: What absorbance value corresponds to 40.2% T? A = A 0.0130 M solution of a certain analyte exhibits…

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Q: A 6.17 x 10- M sample of compound X in a solvent has an absorbance of 0.300 at 528 nm in a 1.000 cm…

A: Answer: This question is based on Beer-Lambert law which is shown below: A=εbC Here:…

Q: 4.00 3.50 3.00 2.50 2.00 1.50 1.00 y = 19883x + 0.5405 R? = 0.8873 0.50 0.00 0.00E+00 5.00E-05…

A: This can be obtained by putting drop line

Q: 2. What is the concentration (mM) of a solution of tryptophan if the absorbance at 280 nm is 0.850?…

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Q: 20 What absorbance value corresponds to 47.4% T? A = 0.323 A 0.0141 M solution of a certain analyte…

A: 1. What absorbance value corresponds to 47.4% Т?A =2. A 0.0141 M solution of a certain analyte…

Q: Answer the following questions based on the UV-Vis spectrum below: (i) (ii) 0.879 - 0.525 Absorbance…

A: (i)

Q: 50 uL of an aqueous sample of double-stranded DNA is dissolved in 950 uL of water. This diluted…

A: GIVEN  Absorbance (A)= 0.326 at 260 nm Molar absorbivity (ε) =0.020 ng-1ml λmax= 260 nm

Q: What is the absorbance, A, of a 0.0170 M solution with a percent transmittance of 46.0%? A = Suppose…

A: Relation between absorbance and percent transmittance ---> A = log(%T) Where A= absorbance…

Q: Review Beer's Law. absorbance of 0.75 at 660 nm. An unknown solution of the same metal ion, on the…

A: Given: Concentration of known sample = 2.5 M The absorbance of known sample = 0.75  The absorbance…

Q: Compund AFK has a maximum absorbance of 650 nm. Its molar absorptivity at 650 is 8400 M−1cm−1 and…

A: A = εcl  A = absorbance  ε = absorptivity coefficient (M-1cm-1)  c = concentration of solution…

Q: The absorption coefficient of bovine immunoglobin (molecular mass= 150,000 Daltons) at 280nm is…

A: Given:Molecular mass= 150,000 Daltons  The absorption coefficient = 210,000Wavelength = 280nmThe…

Q: A 50.0 mg sample was dissolved in acid and then diluted to a total volume of 50.00 mL. If 1.000 mL…

A: In spectrophotometric measurements of analyte concentration, measured absorbance related to the…

Q: Assuming the path length is 1 cm, and the molar absorption ? is 0.108 ml mg-1cm^-1 , what would be…

A: According to Beer Lambert Law, the amount of light which is absorbed by any substance is directly…

Q: Calculate the absorbance for a solution containing a colored species with a percent transmittance…

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Q: What absorbance value corresponds to 49.1% T? A = A 0.0179 M solution of a certain analyte exhibits…

A: Given : % T = 49.1 %

Q: What absorbance corresponds to 99% transmittance? To 0.10% transmittance?

A: If A is the absorbance and T is the transmittance, the relation between absorbance and transmittance…

Q: 5. A 2.50x10-5 M solution of a specific dye has an absorbance value of 1.300 when measured at 525 nm…

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.