A linear DNA molecule was cut with the restriction enzymes EcoRI and BamHI, and the DNA fragments separated by gel electrophoresis, as shown below. Sizes of the restriction fragments are shown on the left in kilobases (kb). kb EcoRI BamHI 4 3 2 1

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1a) How many EcoRI recognition sites are in the DNA molecule?

1, 2, 3 or 4?
 
1b) do the sizes of the DNA fragments from the BamHI digest add up to the same as those of the EcoRI digest? 
 
a) Yes, because the enzymes do the same job: to cut the DNA.
b) Yes, because the same DNA molecule was used in the digests.
c) No, because the fragment sizes produced by the two enzymes are different.
d) No, because the enzymes have different cutting sites.
A linear DNA molecule was cut with the restriction enzymes EcoRI and BamHI, and the DNA
fragments separated by gel electrophoresis, as shown below. Sizes of the restriction
fragments are shown on the left in kilobases (kb).
kb
EcoRI BamHI
4
3
1
CO
Transcribed Image Text:A linear DNA molecule was cut with the restriction enzymes EcoRI and BamHI, and the DNA fragments separated by gel electrophoresis, as shown below. Sizes of the restriction fragments are shown on the left in kilobases (kb). kb EcoRI BamHI 4 3 1 CO
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