THEME I: Please label and rank the following point mutations in increasing order of their potential to change a protein product (assign number 1 to the most benign mutation, if two mutations have the same effect you can assign them the same rank)

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**THEME I: Mutation Analysis and Ranking**

**Objective:** Label and rank the following point mutations in increasing order of their potential to change a protein product. Assign number 1 to the most benign mutation. If two mutations have the same effect, you can assign them the same rank.

---

**Wild type Sequence:**

- **Codons:** AUG GGC TGC AAA CGC TGG
- **Amino Acids:** Met Ala Cys Lys Arg Trp

---

**Mutations:**

**A)**

- **Codons:** AUG GCT TGC AAA CGC TGG
- **Amino Acids:** Met Ala Cys Lys Arg Trp

**B)**

- **Codons:** AUG GCC TGA AAA CGC TGG
- **Amino Acids:** Met Ala --- --- --- 

**C)**

- **Codons:** AUG GCC GGC AAA CGC TGG
- **Amino Acids:** Met Ala Arg Lys Arg Trp

**D)**

- **Codons:** AUG GCC C TGC AAA CGC TGG
- **Amino Acids:** Met Ala Leu Gln Thr Leu

**E)**

- **Codons:** AUG GCC TGC TGC TGC TGC
- **Amino Acids:** Met Ala Cys Cys Cys Cys

---

**Instructions:**

Fill in the type of mutation and ranking based on the potential to change the protein product:

A) Type of Mutation: _________ Rank: _________

B) Type of Mutation: _________ Rank: _________

C) Type of Mutation: _________ Rank: _________

D) Type of Mutation: _________ Rank: _________

E) Type of Mutation: _________ Rank: _________

---

Note: Visual inspection will help you determine the nature and impact of each mutation, which may include substitutions, insertions, or deletions affecting the amino acid sequence and potentially protein function.
Transcribed Image Text:**THEME I: Mutation Analysis and Ranking** **Objective:** Label and rank the following point mutations in increasing order of their potential to change a protein product. Assign number 1 to the most benign mutation. If two mutations have the same effect, you can assign them the same rank. --- **Wild type Sequence:** - **Codons:** AUG GGC TGC AAA CGC TGG - **Amino Acids:** Met Ala Cys Lys Arg Trp --- **Mutations:** **A)** - **Codons:** AUG GCT TGC AAA CGC TGG - **Amino Acids:** Met Ala Cys Lys Arg Trp **B)** - **Codons:** AUG GCC TGA AAA CGC TGG - **Amino Acids:** Met Ala --- --- --- **C)** - **Codons:** AUG GCC GGC AAA CGC TGG - **Amino Acids:** Met Ala Arg Lys Arg Trp **D)** - **Codons:** AUG GCC C TGC AAA CGC TGG - **Amino Acids:** Met Ala Leu Gln Thr Leu **E)** - **Codons:** AUG GCC TGC TGC TGC TGC - **Amino Acids:** Met Ala Cys Cys Cys Cys --- **Instructions:** Fill in the type of mutation and ranking based on the potential to change the protein product: A) Type of Mutation: _________ Rank: _________ B) Type of Mutation: _________ Rank: _________ C) Type of Mutation: _________ Rank: _________ D) Type of Mutation: _________ Rank: _________ E) Type of Mutation: _________ Rank: _________ --- Note: Visual inspection will help you determine the nature and impact of each mutation, which may include substitutions, insertions, or deletions affecting the amino acid sequence and potentially protein function.
Expert Solution
Step 1: Mutation and protein structure :

Label and rank of the given mutations in increasing (smaller to larger) order of their potential to change a protein structure :


A. Silent mutation; smallest change in protein structure. 

C. Missence mutation; small change in protein structure. 

E. Trinucleotide repeat (TNR) expansion; medium change in protein structure. 

D. Frameshift mutation; larger change in protein structure. 

B. Nonsense mutation; largest change in protein structure. 


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