A glucosamine solution of unknown concentration was diluted as follows:  1 mL of the solution was diluted to 5.0  mL.  A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank).    What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equation

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Chapter1: Biochemistry: An Evolving Science
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A glucosamine solution of unknown concentration was diluted as follows:  1 mL of the solution was diluted to 5.0  mL.  A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank).   

What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL?

Given:

Corrected absorbance = 0.395

y= 1.165x+0.055 Standard equation 

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