a) ; Construct a Lineweaver -Burk plot using the kinetic data shown in Table 1.. Determine Vmax and Km for the uninhibited enzyme and inhibited enzyme. .
a) ; Construct a Lineweaver -Burk plot using the kinetic data shown in Table 1.. Determine Vmax and Km for the uninhibited enzyme and inhibited enzyme. .
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
![Table below shows the kinetic data obtained for an enzyme in the absence of any inhibitor (1), and
in the presence of an inhibitor (2), at a concentration of 7.0 mM. Assume the total enzyme
concentration, [E]T, is the same for each experiment.
Table 1
[S]
(mM)
Vo
Vo
{umol/(mL•s)}
{umol/(mL•s)}
Without Inhibitor
With Inhibitor
(1)
3.8
(2)
2.2
1
2
7.3
4.3
4
13.3
8.2
8
22.9
14.9
12
30.0
20.4
a); Construct a Lineweaver -Burk plot using the kinetic data shown in Table 1.
Determine Vmax and Km for the uninhibited enzyme and inhibited enzyme.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa7555c48-a334-459a-ae1f-ef0a9e775694%2F76594c60-2df0-4af4-b4ff-d4d0a1b33d26%2Fsdig6d_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Table below shows the kinetic data obtained for an enzyme in the absence of any inhibitor (1), and
in the presence of an inhibitor (2), at a concentration of 7.0 mM. Assume the total enzyme
concentration, [E]T, is the same for each experiment.
Table 1
[S]
(mM)
Vo
Vo
{umol/(mL•s)}
{umol/(mL•s)}
Without Inhibitor
With Inhibitor
(1)
3.8
(2)
2.2
1
2
7.3
4.3
4
13.3
8.2
8
22.9
14.9
12
30.0
20.4
a); Construct a Lineweaver -Burk plot using the kinetic data shown in Table 1.
Determine Vmax and Km for the uninhibited enzyme and inhibited enzyme.
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