The enzyme catalase catalyzes a reaction that breaks down hydrogen peroxide (H₂O2), atoxic chemical that is produced as a by-product of many cellular reactions, into two harm-less substances: water and oxygen. The reaction is shown below.→ 2H₂O + O₂2H₂O2This reaction is important to cells because hydrogen peroxide is a by-product of many nor-mal cellular reactions.(a) An experiment can be designed to test the effect of different temperature levels on thefunctioning of catalase. Identify the appropriate independent and dependent variables forthis experiment.(b) Identify an appropriate method to measure the dependent variable in this experimentand justify your choice.Respond in 4 to 6 complete sentences.

The independent variable is the parameter which we expect to affect the dependent variable. The…

Answered: (a) An experiment can be designed to…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

A newly developed drug is suspected to act by inhibiting its enzymatic target. To test this, the rates of reaction in the presence and absence of the new drug were determined. The results of these experiments have been plotted as a double reciprocal plot shown in figure 1 below. Using this figure, calculate the values of Vmax and Km for the enzyme in the presence and absence of the drug. 2. Based on the figure and your answer, what type of inhibition is the new drug displaying? 3. Briefly explain how this type of inhibition exerts its action on an enzyme. Figure 1: Double reciprocal plot of reaction rate against substrate concentration in the presence and absence of a new drug.
Which question will your experiment investigate? (select one and delete the others) How does temperature affect how lactase drops work to break down lactose into glucose and galactose? How does pH affect how lactase drops work to break down lactose into glucose and galactose? How does agitation (movement) affect how lactase drops work to break down lactose into glucose and galactose? What variable will you change in this experiment? This is also called the Independent variable. How will you change it? What variable will you measure in this experiment to determine the effect of the variable you changed? This is also called the Dependent variable. How will you measure it? What variables will you keep the same in this experiment? Check off the items in this list that you would use for this experiment. You may add any items you feel you would need. O Skim milk (which contains lactose) O Lactase drops O Graduated cylinder O Eye dropper O Ice cubes O pH paper O Hot plate O Mixing spoon O…
You design an enzyme assay and choose a substrate concentration equal to the Km of the enzymatic reaction. You measure the rate of the reaction at this substrate concentration to be 75.0 μmol/min. Calculate the Vmax of this reaction in μmol/min. Express your answer with one decimal place. Your previous attempt suffered from low signal-to-noise ratio when you used a substrate concentration equal to the Km. So you decide to increase the substrate concentration to 0.45 mM. You remeasure the rate of the reaction at this new substrate concentration and find it to be 135.0 μmol/min. Using the same Vmax value found above, calculate the Km value of this enzymatic reaction. Express your answer in μM with one decimal place. Considering the previous two questions, what would be the rate of the reaction at a substrate concentration of 0.01 mM? Express your answer in μmol/min with one decimal place.
1, For EACH of the following enzyme categories, (1) describe what the general function of the enzyme is; (2) identify a SPECIFIC enzyme in glycolysis that performs this function; and (3) identify the names of the organic substrate and product for the specific enzyme identified. Remember that the name of the category need not appear in the actual name of the enzyme. A. First enzyme category: kinase (answer (1), (2), and (3) B. Second enzyme category: dehydrogenase (answer (1), (2), and (3) C. Third enzyme category: isomerase (answer (1), (2), and (3)
You are given the task of determining the approximate % solute of potato cells. You decide to set up a series of 10 cups ranging from dH2O to 1% sucrose solution. You intend to drop a potato stick in each cup. a. What data will you collect for each potato stick? b. In how many of the solutions will the potato sticks remain unchanged? c.
A protein (PrX) was heated in vitro to 45C for 15 min, in the presence or absence of Hsp40, Hsc70, and ATP. The amount of PrX in aggregated (P, pellet) and soluble (S) fractions was then quantified (Bars, 1 SD). Based on these data, answer the following questions. In all cases, give brief justification for your answer. a) How does Hsp40 alone influence the solubility of PrX? b) How does Hsc70 alone influence the solubility of PrX? c) Do Hsp40 and Hsc70 co-operate in this experiment? d) Which components combined provide maximal solubility of PrX after heat shock?
Intestinal epithelial cells pump glucose into the cell against its concentration gradient using the Na*-glucose symporter. Recall that the Na+ concentration is significantly higher outside the cell than inside the cell. The symporter couples the "downhill" transport of two Na+ ions into the cell to the "uphill" transport of glucose into the cell. If the Na+ concentration outside the cell ([Na* lout) is 147 mM and that inside the cell ([Na+]in) is 17.0 mM, and the cell potential is -54.0 mV (inside negative), calculate the maximum energy available for pumping a mole of glucose into the cell. Assume the temperature is 37 °C. AG gluc kJ mol What is the maximum ratio of [glucose]in to [glucose] out that could theoretically be produced if the energy coupling were 100% efficient? 1.13 2.3 × 10-4 8.36 4300

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS