9. Your lab partner has followed the Gram staining protocol on two bacterial samples, Escherichia coli and Bacillus subtilis. What color stain and Gram stain results would they expect to see? If both sample results are stained pink, what conclusion would you make? If this is not the expected result, what could you change to get the desired results?

9. Your lab partner has followed the Gram staining protocol on two bacterial samples, Escherichia coli and Bacillus subtilis. What color stain and Gram stain results would they expect to see? If both sample results are stained pink, what conclusion would you make? If this is not the expected result, what could you change to get the desired results?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 2RQ

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Similar questions

1.why is gram stain considered a differential stain? 2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?
1) In Gram staining, to what cell structure do the dyes bind? 2) Would it be useful to perform a Gram stain on a mixed culture? Why?
5) Which is the most crucial step in the Gram staining procedure? Why?
1) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step during the staining procedure. 2) What is a gram variable and why can gram variability occur?
Briefly, in your own words, describe the steps of the gram staining procedure.
Why is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.
Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)
Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorization
Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that step affect the end result?
A mixed culture is known to contain Gram negative bacilli and Gram positive cocci. You perform a Gram stain of this mixed culture as a control while simultaneously Gram stainin an unknown organism on the same slide (the same procedure you used in lab). The results, observed under the 100x objective lens, are described below: Results: Control side: purple bacilli and purple cocci Unknown side: purple bacilli Using all the information given above, discuss the results of your unknown organism. Shoul you be confident in this result? Answer question A if you are confident, answer question B if you are not confident. a. If you are confident in your results, why? Explain how the staining reagents produced the described results. b. If you are not confident, do you think an error was made in the Gram staining procedure? If so, describe the error and explain how it impacted your results.
After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?
ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?