A patient comes into a hospital and has what appears to be an infection. Your microscope is broken so staining is not an option. Design an experiment to determine if the infection is caused by gram positive or gram negative bacteria.
A patient comes into a hospital and has what appears to be an infection. Your microscope is broken so staining is not an option. Design an experiment to determine if the infection is caused by gram positive or gram negative bacteria.
Chapter5: Phlebotomy Equipment
Section: Chapter Questions
Problem 4.BE
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A patient comes into a hospital and has what appears to be an infection. Your microscope is broken so staining is not an option. Design an experiment to determine if the infection is caused by gram positive or gram negative bacteria.
Transcribed Image Text:**Experiment Design: Identifying Gram-Positive vs. Gram-Negative Bacteria**
A patient arrives at a hospital with a suspected infection. The laboratory microscope is inoperable, so traditional staining methods cannot be used. Your task is to design an experiment to determine whether the infection is caused by a gram-positive or gram-negative bacterium. Consider alternative techniques, such as molecular methods, biochemical tests, or antibiotic susceptibility profiles. Document your approach, expected results, and potential limitations in your design.
**Key Considerations:**
1. **Method Selection**: Choose a method that does not require microscopy or staining.
2. **Biochemical Testing**: Utilize biochemical tests that differentiate between bacterial wall structures.
3. **Molecular Techniques**: Consider PCR or other DNA-based methods to identify bacterial DNA or specific genetic markers.
4. **Antibiotic Profiles**: Analyze the bacteria's resistance or sensitivity to specific antibiotics known to target gram-positive or gram-negative bacteria.
5. **Ethical Considerations**: Ensure patient safety and data confidentiality throughout the experimentation process.
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