9. On the SDS-PAGE gel below below, which sample is largest? 3 ample Sample 1 Sample 3 Sample 2 SDS-PAGE

Table I. Sakaguchi Reaction Samples 1. Arginine 2. Casein 3. Albumin 4. Gelatin 5. Peptone 6. distilled water Observation

7. Short answer. Professor Forni has hired vou as a research assistant and you need to make an X-gal staining solution for your sections containing 5mM potassium ferrocyanide, 5 mM potassium ferricyanide, 2 MM MgCl2, 0.1% Tween and 0.1% BCIP. You need 50 ml. As you are getting ready you discover that your colleague has used most of the BCIP and you only have 150 ul of the BCIP left. Is it enough? Show your calculations. 8. Short answer. Professor Scimemi has accepted vou as a Master's student and you are involved in a project that studies the cellular basis of neuropsychiatric diseases. For your electrophysiology recordings you have to make a recording solution containing (in mM): 119 NaCI, 2.5 KCI, 2.5 CaCl2, 1 MgCl2, 26.2 NaHCO3, 1 NaH¿PO4, 22 glucose. You need 800 ml of it. How much NaCl and how much KCl do you need to weigh out? (MW NaCI is 58.44 g/mol; MW of KCI is 74.55 g/mol) 9. You are working as a research technician for Pfizer and in your lab, one solution of hydrochloric…

File guft 1.yt Machine Cochise14ee7 Lane 0 Pmer. DTarPOPD mob Comment 17703 Spacing 15.06 Siga C 17A 4 G 2 Bases 0 an 2004 Gelstat ime123 219 20 30 60 70 NNACTCA TCTOGTGGA TTC CTA TCCTG AC A AG TGATGTG CAAAC TG GTAACTC TG AG GCAGATAAC CA G GG CA AA AAGGTG TATAAG 100 110 140 100 CAGA AG TC CGGA AA A TCA TT TAA 170 TAAA ACA AAGCCCTAACT TG GAAG AAGT TCA GTTTTACACA TCT TTA TA TG GAGAGAA 180 TAT TCTAT TA ATOTCCTGT TA TATT TG TCA TATTCA TA CAGT TGTCACAGTATATT TCAAAC CA AC TG TTTAAAA ACAAAC TO AAATAAA 210 230 240 260 270 AAATTTAAATACCCT TA TG TA AA ATAG GCT TC CC TG GTG GCTCAG GG GTAAAAA ACTCGC CCGC CA ACGCAG GAGATGTAGAT TTGATC CCT 300 310 340 350 410 430 440 GG GT TAG GA AG icCCTa GAGA AGGAAATGAAAAAC CACTCTAG TAT TCT Tac CTG GG AAATC CCA TGGACAG AG GAGCOTO GAG G Gc 490 500 SI0 530 TACAGTCCATGGGAGTCGCAA A AGAGT TG GACATG ACTAA ACA ACAACATATAAAATAACCT TACTC CATAATGTCAAACT TATOTCACAC S40 AAA ATGCAA AGT TCT TACATCTAT TAACTTTTATGOT TAAATATA ACCTAATGCACTOTTT TATACAGCA ACAACTACT TT TT TATTT TAAA…

5. RX: Peppermint spirit 2ml Alcohol 10ml Sterile water QS 100ml Approximately how many fluid ounces of sterile water are needed to compound this solution?

3. You were instructed to add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL of sterile diluent. Instead, you add all 5.0 mL to the 125.25 mL, What was the intended dilution and what was the actual dilution? ited States) E Focus OCT 30 W MacBook P DII DD F7 F8 F9 F10 F4 F5 F6 F3 2$ 4 081 R F G H. K < CO

You have to prepare an agarose solution for gel electrophoresis. How many grams of agarose powder do you need in order for you to make 50mL of 1% agarose solution?

The following chart contains four facets. Each shows the amount of a substance in different conditions: • when the cells are adhering to a surface • when the cells are growing in suspension for different amounts of time There are 1 different glyphs shown in the frames. suspension growth suspension growth 1433B 1433E 6.000% 4.000% 16.000% 10.666% 2.000% 5.333% 0.000% 0.000% 1433G 1433Z 4.500% 6.000% 3.000% 4.000% 1.500% 2.000% 0.000% 0.000% adhesion growth O min * 10 min 30 min 60 min growth O min uoisaupe 10 min 30 min 60 min

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Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel? 2. What is a gradient gel? Why are we using them? 3. Compare and contrast SDS-PAGE and native PAGE. Why would we want to do each of these techniques?