8. Altering Linking Number (a) What is the Lk of a 5,000 bp circular duplex DNA molecule with a nick in one strand? (b) What is the Lk of the molecule in (a) when the nick is sealed (relaxed)? (c) How would the Lk of the molecule in (b) be affected by the action of a single molecule of E. coli topoisomerase I? (d) What is the Lk of the molecule in (b) after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP? (e) What is the Lk of the mol- ecule in (d) after four enzymatic turmovers by a single mole- cule of bacterial type I topoisomerase? () What is the Lk of the molecule in (d) after binding of one nucleosome?

8. Altering Linking Number (a) What is the Lk of a 5,000 bp circular duplex DNA molecule with a nick in one strand? (b) What is the Lk of the molecule in (a) when the nick is sealed (relaxed)? (c) How would the Lk of the molecule in (b) be affected by the action of a single molecule of E. coli topoisomerase I? (d) What is the Lk of the molecule in (b) after eight enzymatic turnovers by a single molecule of DNA gyrase in the presence of ATP? (e) What is the Lk of the mol- ecule in (d) after four enzymatic turmovers by a single mole- cule of bacterial type I topoisomerase? () What is the Lk of the molecule in (d) after binding of one nucleosome?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

8. Altering Linking Number (a) What is the Lk of a 5,000
bp circular duplex DNA molecule with a nick in one strand?
(b) What is the Lk of the molecule in (a) when the nick is
sealed (relaxed)? (c) How would the Lk of the molecule in
(b) be affected by the action of a single molecule of E. coli
topoisomerase I? (d) What is the Lk of the molecule in (b)
after eight enzymatic turnovers by a single molecule of DNA
gyrase in the presence of ATP? (e) What is the Lk of the mol-
ecule in (d) after four enzymatic turnovers by a single mole-
cule of bacterial type I topoisomerase? () What is the Lk of
the molecule in (d) after binding of one nucleosome?

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