5'GGT ACG TTG GGG CTC CAT3' This sequence is transcribed and translated. If the G that is underlined changes to to a C the result will be A) A nonsenese mutation. B) A frameshift mutation C) A silent substitution. D) A missense mutation

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**Genetic Mutations and Genetic Code Analysis**

**1. Sequence Mutation Analysis:**

**Sequence:**
5’GGT ACG TTG GGG CTC CAT3’

This sequence is transcribed and translated.

**Question 1:**
If the G that is underlined changes to a C, the result will be:

- A) A nonsense mutation
- B) A frameshift mutation
- C) A silent substitution
- D) A missense mutation

**Question 2:**
If the G that is underlined is deleted, the result will be:

- A) A nonsense mutation
- B) A frameshift mutation
- C) A silent substitution
- D) A missense mutation

**2. Gene Length and Protein Analysis:**

**Question:**
If there are 3000 bases in the coding region of a gene, the gene will have:

- A) 3000 amino acids
- B) 6000 amino acids
- C) 1000 amino acids
- D) 3000 codons

---

**Explanation of Concepts:**

- **Nonsense Mutation:** A mutation that introduces a premature stop codon, leading to truncated proteins.
- **Frameshift Mutation:** Caused by insertions or deletions that change the reading frame of the genetic sequence.
- **Silent Substitution:** A mutation that does not change the encoded amino acid.
- **Missense Mutation:** A mutation that results in a different amino acid being incorporated into the resulting protein.
- **Codons:** Groups of three bases in mRNA that code for a specific amino acid.
- **Amino Acids:** Building blocks of proteins; typically, one codon corresponds to one amino acid. In a typical gene, each set of three nucleotides (a codon) codes for one amino acid. Thus, 3000 bases would normally translate into 1000 amino acids.
Transcribed Image Text:**Genetic Mutations and Genetic Code Analysis** **1. Sequence Mutation Analysis:** **Sequence:** 5’GGT ACG TTG GGG CTC CAT3’ This sequence is transcribed and translated. **Question 1:** If the G that is underlined changes to a C, the result will be: - A) A nonsense mutation - B) A frameshift mutation - C) A silent substitution - D) A missense mutation **Question 2:** If the G that is underlined is deleted, the result will be: - A) A nonsense mutation - B) A frameshift mutation - C) A silent substitution - D) A missense mutation **2. Gene Length and Protein Analysis:** **Question:** If there are 3000 bases in the coding region of a gene, the gene will have: - A) 3000 amino acids - B) 6000 amino acids - C) 1000 amino acids - D) 3000 codons --- **Explanation of Concepts:** - **Nonsense Mutation:** A mutation that introduces a premature stop codon, leading to truncated proteins. - **Frameshift Mutation:** Caused by insertions or deletions that change the reading frame of the genetic sequence. - **Silent Substitution:** A mutation that does not change the encoded amino acid. - **Missense Mutation:** A mutation that results in a different amino acid being incorporated into the resulting protein. - **Codons:** Groups of three bases in mRNA that code for a specific amino acid. - **Amino Acids:** Building blocks of proteins; typically, one codon corresponds to one amino acid. In a typical gene, each set of three nucleotides (a codon) codes for one amino acid. Thus, 3000 bases would normally translate into 1000 amino acids.
**DNA Sequence Transcription and Translation Exercise**

**Sequence 1:**

5’GGT ACG TTG GGG CTC CAT3’

This sequence is transcribed and translated. Write the resulting amino acid sequence using the 3-letter code. Write the answer in all capital letters. Leave a space between the amino acids. Do not write 5' and 3'.

[__________]

---

**Mutation Analysis:**

**Original Sequence:**

5’GGT ACG TTG GGG CTC CAT3’

This sequence is transcribed and translated.

**1. Mutation Scenario 1:**

If the G in bold changes to a T, then the result will be:

- ( ) A) A nonsense mutation
- ( ) B) A frameshift mutation
- ( ) C) A silent substitution
- ( ) D) A missense mutation

**2. Mutation Scenario 2:**

If the G in bold changes to an A, then the result will be:

- ( ) A) A nonsense mutation
- ( ) B) A frameshift mutation
- ( ) C) A silent substitution
- ( ) D) A missense mutation

---

**Instructions:**

Explore the potential impacts of point mutations in this DNA sequence. Consider how changes at specific sites might affect the resulting protein.
Transcribed Image Text:**DNA Sequence Transcription and Translation Exercise** **Sequence 1:** 5’GGT ACG TTG GGG CTC CAT3’ This sequence is transcribed and translated. Write the resulting amino acid sequence using the 3-letter code. Write the answer in all capital letters. Leave a space between the amino acids. Do not write 5' and 3'. [__________] --- **Mutation Analysis:** **Original Sequence:** 5’GGT ACG TTG GGG CTC CAT3’ This sequence is transcribed and translated. **1. Mutation Scenario 1:** If the G in bold changes to a T, then the result will be: - ( ) A) A nonsense mutation - ( ) B) A frameshift mutation - ( ) C) A silent substitution - ( ) D) A missense mutation **2. Mutation Scenario 2:** If the G in bold changes to an A, then the result will be: - ( ) A) A nonsense mutation - ( ) B) A frameshift mutation - ( ) C) A silent substitution - ( ) D) A missense mutation --- **Instructions:** Explore the potential impacts of point mutations in this DNA sequence. Consider how changes at specific sites might affect the resulting protein.
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