4. Determination of sodium and potassium in blood or serum by low temperature flame emission on simple instruments is complicated by the instability of the flame and the complexity of the matrix. One way to overcome the problems is to add an excess of lithium to serve as an internal standard. To be a good internal standard, lithium emission responds similarly to the emissions of Na and K to changes in the flame conditions. The responses found for calibration solutions shown in the top table are listed in the bottom table. Concentration (ppm) Sample Na K Li A 0.5 0.5 500 B 1.0 1.0 500 C 5.0 5.0 500 D 10.0 10.0 500 Signal (relative to Na in 1.0 ppm Na sample) Sample Na K Li A 0.43 0.57 31.2 B 1.00 1.25 36.3 с 4.9 6.4 36.2 D 8.8 11.7 31.7 (a) Generate the calibration plots for Na and K with Li as internal standard. (b) A blood sample of 10.0 μL was diluted in 2.00 mL of 500 ppm Li solution and was fed into the spectrometer. The readings were Na =13.6, K=1.34, and Li = 30.4. What are the serum concentrations of Na and K in mM? Assume Na and K do not interfere with each other.
4. Determination of sodium and potassium in blood or serum by low temperature flame emission on simple instruments is complicated by the instability of the flame and the complexity of the matrix. One way to overcome the problems is to add an excess of lithium to serve as an internal standard. To be a good internal standard, lithium emission responds similarly to the emissions of Na and K to changes in the flame conditions. The responses found for calibration solutions shown in the top table are listed in the bottom table. Concentration (ppm) Sample Na K Li A 0.5 0.5 500 B 1.0 1.0 500 C 5.0 5.0 500 D 10.0 10.0 500 Signal (relative to Na in 1.0 ppm Na sample) Sample Na K Li A 0.43 0.57 31.2 B 1.00 1.25 36.3 с 4.9 6.4 36.2 D 8.8 11.7 31.7 (a) Generate the calibration plots for Na and K with Li as internal standard. (b) A blood sample of 10.0 μL was diluted in 2.00 mL of 500 ppm Li solution and was fed into the spectrometer. The readings were Na =13.6, K=1.34, and Li = 30.4. What are the serum concentrations of Na and K in mM? Assume Na and K do not interfere with each other.
Chapter8: Sampling, Standardization, And Calibration
Section: Chapter Questions
Problem 8.21QAP
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Transcribed Image Text:4.
Determination of sodium and potassium in blood or serum by low temperature flame
emission on simple instruments is complicated by the instability of the flame and the
complexity of the matrix. One way to overcome the problems is to add an excess of
lithium to serve as an internal standard. To be a good internal standard, lithium emission
responds similarly to the emissions of Na and K to changes in the flame conditions. The
responses found for calibration solutions shown in the top table are listed in the bottom
table.
Concentration (ppm)
Sample
Na
K
Li
A
0.5
0.5
500
B
1.0
1.0
500
C
5.0
5.0
500
D
10.0
10.0
500
Signal (relative to Na in 1.0 ppm Na sample)
Sample
Na
K
Li
A
0.43
0.57
31.2
B
1.00
1.25
36.3
с
4.9
6.4
36.2
D
8.8
11.7
31.7
(a) Generate the calibration plots for Na and K with Li as internal standard.
(b) A blood sample of 10.0 μL was diluted in 2.00 mL of 500 ppm Li solution and was
fed into the spectrometer. The readings were Na =13.6, K=1.34, and Li = 30.4. What
are the serum concentrations of Na and K in mM?
Assume Na and K do not interfere with each other.
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