Pls explain the steps on why it came to be because I have a hard understanding this lesson T - T You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine its amino acid sequence. The amino acid composition is K2, D, Y, F, G, S, A. Reaction of the intact peptide with dansyl chloride yields dansyl - A. Cleavage with trypsin yields peptides whose compositions are: (K, A, S) and (G, F, K) plus a dipeptide. Reaction with chymotrypsin releases free D, a tetrapeptide with composition (k, S, F, A) and a tripeptide whose composition following acid hydrolysis is (G, K, Y). The enzymatic digests are each carried out on the whole, undansylated peptide. What is the sequence?
Pls explain the steps on why it came to be because I have a hard understanding this lesson T - T You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine its amino acid sequence. The amino acid composition is K2, D, Y, F, G, S, A. Reaction of the intact peptide with dansyl chloride yields dansyl - A. Cleavage with trypsin yields peptides whose compositions are: (K, A, S) and (G, F, K) plus a dipeptide. Reaction with chymotrypsin releases free D, a tetrapeptide with composition (k, S, F, A) and a tripeptide whose composition following acid hydrolysis is (G, K, Y). The enzymatic digests are each carried out on the whole, undansylated peptide. What is the sequence?
Chapter26: Biomolecules: Amino Acids, Peptides, And Proteins
Section26.SE: Something Extra
Problem 28MP
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Transcribed Image Text:Pls explain the steps on why it came to be because I have a
hard understanding this lesson T - T You have isolated from a
rare fungus an octapeptide that prevents baldness and you
wish to determine its amino acid sequence. The amino acid
composition is K2, D, Y, F, G, S, A. Reaction of the intact
peptide with dansyl chloride yields dansyl - A. Cleavage with
trypsin yields peptides whose compositions are: (K, A, S) and
(G, F, K) plus a dipeptide. Reaction with chymotrypsin
releases free D, a tetrapeptide with composition (k, S, F, A)
and a tripeptide whose composition following acid hydrolysis
is (G, K, Y). The enzymatic digests are each carried out on
the whole, undansylated peptide. What is the sequence?
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