Pls explain the steps on why it came to be because I have a hard understanding this lesson T - T You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine its amino acid sequence. The amino acid composition is K2, D, Y, F, G, S, A. Reaction of the intact peptide with dansyl chloride yields dansyl - A. Cleavage with trypsin yields peptides whose compositions are: (K, A, S) and (G, F, K) plus a dipeptide. Reaction with chymotrypsin releases free D, a tetrapeptide with composition (k, S, F, A) and a tripeptide whose composition following acid hydrolysis is (G, K, Y). The enzymatic digests are each carried out on the whole, undansylated peptide. What is the sequence?

Organic Chemistry
9th Edition
ISBN:9781305080485
Author:John E. McMurry
Publisher:John E. McMurry
Chapter26: Biomolecules: Amino Acids, Peptides, And Proteins
Section26.SE: Something Extra
Problem 28MP
icon
Related questions
Question
Please correct answer and don't use hand rating
Pls explain the steps on why it came to be because I have a
hard understanding this lesson T - T You have isolated from a
rare fungus an octapeptide that prevents baldness and you
wish to determine its amino acid sequence. The amino acid
composition is K2, D, Y, F, G, S, A. Reaction of the intact
peptide with dansyl chloride yields dansyl - A. Cleavage with
trypsin yields peptides whose compositions are: (K, A, S) and
(G, F, K) plus a dipeptide. Reaction with chymotrypsin
releases free D, a tetrapeptide with composition (k, S, F, A)
and a tripeptide whose composition following acid hydrolysis
is (G, K, Y). The enzymatic digests are each carried out on
the whole, undansylated peptide. What is the sequence?
Transcribed Image Text:Pls explain the steps on why it came to be because I have a hard understanding this lesson T - T You have isolated from a rare fungus an octapeptide that prevents baldness and you wish to determine its amino acid sequence. The amino acid composition is K2, D, Y, F, G, S, A. Reaction of the intact peptide with dansyl chloride yields dansyl - A. Cleavage with trypsin yields peptides whose compositions are: (K, A, S) and (G, F, K) plus a dipeptide. Reaction with chymotrypsin releases free D, a tetrapeptide with composition (k, S, F, A) and a tripeptide whose composition following acid hydrolysis is (G, K, Y). The enzymatic digests are each carried out on the whole, undansylated peptide. What is the sequence?
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9781305080485
Author:
John E. McMurry
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9781305580350
Author:
William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:
Cengage Learning