Calculate the theoretical isoelectric point of the following peptide. OH CH, CH, CH H,N CH₂OH H CH₂ H CH, CH₂ -N- -C-N-C-COO H o H ° H 0 H ° H Amino acid PK1 (-COOH) pK2 (-NH2) PKR Mr Leucine 2.36 9.6 131 Glycine 2.34 9.6 75 Lysine 2.18 8.95 10.53 146 Alanine 2.34 9.69 89 Serine 2.21 9.15 105 Tyrosine 2.20 9.11 10.07 181 131 Isoleucine 2.36 9.68 A. 5.76 B. 5.24 OC. 9.61 OD. 6.22 E. 6.45

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