**Table Analysis for DNA Base Composition**The table below represents the base composition ratios from two different double-stranded DNA sources and their RNA products measured in experiments conducted *in vitro*. | Species | A+T/G+C | A+U/G+C | A+G/C+U ||------------------|---------|---------|---------|| *Bacillus subtilis* | 1.36 | 1.30 | 1.02 || *E. coli* | 1.00 | 0.98 | 0.80 |**Discussion Points:**- **Determining RNA Copying Source:** - Examine the given ratios to assess if RNA is copied from a single DNA strand or both strands. Consider if the ratios imply a skewed composition that suggests single-strand transcription.- **Identifying RNA Strand Type:** - Evaluate how the DNA and RNA base composition ratios provide insights into whether the RNA is single or double-stranded. Analyze if near-equal ratios suggest double-stranded RNA or if varied ratios indicate a single strand.

Ervin Chargaff discovered the Base proportion in double stranded DNA. The findings in his study came…

Answered: 3. The data in the following table…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True False
You have PGFPuv at 75ng / uL You need 1.15 ng total DNA in a final volume of 30 . Calculate how much DNA and water you will need to add to both your Enzyme Mix and your Control Mix and fill out the table below.
We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transduction
Which of the following functions are associated with the E. coli RNA polymerase? Select all that apply Helicase activity Topoisomerase activity 3' to 5' polymerase activity 5' to 3' polymerase activity
A small section of bacterial DNA template (anti-sense) strand has the following nucleotide sequence: AAG TAT TAT GCA A mutation in the above sequence involved the insertion of a single base, leading to a shift in the reading frame of the gene, resulting in altered amino acids downstream from the insertion. Which of the following gene sequences exemplifies the mutation described above? a. AAG CTA TTA TGC A b. AG TAT TAT GCA c. AAG TATU TAT GCA d. AAG TAT TAGT GCA
Restriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…
What is the consensus sequence of these three DNA sequences? 5' - G G T G C A C - 3'5' - A A T T C A C - 3'5' - G G T T C T C - 3'
The E. coli genome has a superhelical density in vivo of about 0.06. Assuming the DNA has 10.5 bp/turn, what is the expected writhing number of the E. coli genome?
From an Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the transfer origin of each Hfr strain is shown in Figure 1. You want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (Rifs) and thi*. Conjugation experiments are performed between each of the Hfr strains and an F strain Rif Thi™. 0 T leu 10 20 T nadD pyrC trp 40 T his 60 70 2) The results are shown in the following table: Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 cysG 80 90 1) What is the selection medium used in these conjugation experiments metA Colonies Thi+ 1000 0 400 0 25 100 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are shown. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan…
2. pBR3322, an artificially made plasmid, is shown below. ECORI Hindill BamHI Pstl Amp ORI ↓↓ Tetr pBR322 (4.36 kb) ↑ Pvull Xmalll Describe 3 features that this plasmid has and indicate each one on the diagram. i) ii) 111]
Use the plasmid map for pBashi below to answer the following questions Xhol Hind!!! 15 kb 11 kb 10 kb 8 kb 7 kb 5 kb a. How large is pBashi? b. On the gel below, draw the bands corresponding to the expected pBashi cleavage products after restriction digest with the indicated restriction enzymes (Lane 2 - Xhol only, Lane 3 - HindIII only, Lane 4 - Xhol+ HindIII) 4 kb 3 kb 3 kb 2 kb 1 kb 4 kb Hindill 1 kb 7 kb Xhol Xhol Xhol Xhol Hind!!! Ladder Xhol Hindill Hind!!! Pstl Pstl c. Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ Pstl. Lane 6 in the gel above displays the pBashi cleavage products after restriction digest with the restriction enzymes Xhol+ HindIII + Pstl. Indicate your final answers to the questions below clearly on the pBashi plasmid above; you will NOT receive any credit if you only list sizes of fragments below. If you need to, draft your answer somewhere else before writing your final answer above.…
PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?

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