3. Identify the IMVIC pattern of the following tubes. For set of tubes that is E. coli positive, identify if the reaction is typical or atypical. IMVIC Pattern: E. coli positive? Yes I No If yes, type of reaction:
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- 1. To test the ability of each diluent to sustain the viability of Escherichia coli during dilution, the cells were incubated at room temperature (20 to 23°C) or 37°C for 0 or 2 h and plated on NA agarin duplicate. The counts obtained are shown in the following table: E. coli counts on dilution time O hour 2 hours Diluents 10-5 10-6 10-7 10-5 10-6 10-7 R1 26 ; 27 3:2 0:0 0:0 0:0 0:0 Distilled Water R2 31 ; 27 1:0 0:0 0:0 0:0 0:0 (DW) R3 27 : 28 4:5 0:0 0:0 0:0 0:0 R1 290 : 295 34 :31 2:1 252 : 239 27 : 26 1;1 DW-0.85% NacI R2 315 ; 309 37 : 34 2;2 250 : 245 26 ; 26 1:0 R3 301 ; 285 36 : 44 1:2 235 ; 267 34 : 21 0:0 R1 385 ; 392 42 : 35 4:5 368 :379 33 ; 32 2:2 DW-3.0% Nacı R2 370 : 388 37 : 40 2:4 305 : 303 35 ; 30 2:5 R3 355 : 365 35 : 28 4:6 370 :350 33: 37 3:1 R1 380 ; 383 30 : 27 2:2 255 : 245 25 : 24 2;2 PBS R2 341 ; 352 27 : 34 1:3 230 : 245 26 : 27 2:2 R3 329 ; 335 33 : 31 3:6 242 : 250 28 : 29 2:1 R1 340 ; 320 32 : 35 1:0 245 : 249 27 : 25 1 1 PBS-0.85% Naci R2 340 ; 345 35 :…t /g = (Log Nt – Log N0) /0.301 I introduce a loopful of Escherichia coli cells (say, 1000) into 10 mL of Nutrient Broth at 8 p.m. the night before your lab. The cells were taken from a culture plate (Nutrient Agar) held at 37°C, and inoculated into broth at the same temperature. They were held at 37°C overnight in a shaking water bath. At what time would the culture reach the Stationary Phase? Recall that doubling time under optimal conditions (these are) is 20 minutes. A growing bacterial culture has 10,000 CFU/mL at noon and 10,000,000 CFU/mL at 6 p.m. What is the generation time under these conditions? What are your assumptions? At midnight you inoculate 10 mL of a culture of Enterococcus with 103 cells/mL into 990 mL of the same medium, held under the same conditions as the original culture. At what time would the culture reach 107 cells/mL? Assume exponential growth over the period. Assume that g=half an hour. Note: We worked a different variant of this problem in…A Photo credit: BSP3D- Group 5 Pharm. Bio. Sci 3 Laboratory (S.Y. 2016-2017) Sample is Bacillus subtilis; A-Ciprofloxacin; B-Ampicillin; C-Tetracycline; D- Vancomycin Refer to the image above and answer the following questions: 1. By just looking at the example above, which do you think is the most effective antibiotic for Bacillus subtilis? which is the least effective? and why? 2. How will you measure the zone of inhibition of vancomycin (letter D)? 3. What is the importance of evenly distributing the microbe onto the plate? 3. Subtilis
- 1. Determine the minimum inhibitory concentration and the minimum exposure time given the following data: Number of colonies of E. coli on Nutrient Agar plates Exposure time (in minutes) Concentration of ethanol 10 15 Distilled water +++ +++ +++ 40% +++ ++ + 70% 95% Minimum inhibitory concentration: Minimum exposure time:WT E. coli B Lysate titer on B K pfu/ml 10⁹ 10⁹ 0.1 m.u. 0.2 m.u. 0.5 m.u. 1.0 m.u. rll#x 2.0 m.u. E. coli B Lysate titer on B K 10⁹ 103 ril#y E. coli B Lysate titer on B 10⁹ K 10² rll#x E. coli B Mutations rll#x and rll#y are separated by how many map units? Lysate titer on ril#y B K 10⁹ 1071. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies
- . A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14Below is an image of a western blot and coomassie stain. Need help. I need to know which snack bar (Helfy Bar or RganixSnax) has gluten present. And i need a detailed explanation based on the two tests, as to why the images prove that the product contains gluten.
- Kligler’s Iron Agar:Q10) Give me the expected results for Pseudomonas aeroginosa, Salmonella typhimurium, and Escherichia coli. Pseudomonas aeroginosa, Salmonella typhimurium Escherichia coli.Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН НWhat is the result of this biochemical test in the gelatin deep? Which enzyme is this testing for? What tool is used to inoculate this medium? Gelatin positive liquid, use needle, protease,