Q: How might storage of the coconut water samples at room temperature after collection and before…
A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…
Q: How would you draw out a step in an experiment that included the following? Please help, I am so…
A: Horizontal gene transfer (HGT) is largely responsible worldwide for increasing the incidence of…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…
Q: Briefly describe the main scientific principle(s) underlying the following techniques used in the…
A: Ammonium Sulphate Precipitation: Ammonium sulphate precipitation is a typical method for…
Q: There are so many microbes in a single mL of culture, it is very difficult to perform one dilution…
A: The microbiology studies about both the diseases causing microbes and beneficiary microbes, about…
Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…
A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…
Q: 1. Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo…
A: Introduction In Virology, A Plaque-forming Unit (PFU) Is A Metric That Describes How Many Virus…
Q: Why do you think rapid methods so helpful to identify pathogens microbes in clinical settings. Why…
A: Introduction: Pathogens are the microorganisms which has an ability to cause disease. Mainly…
Q: Which of the following factors is NOT significant in determining the effectiveness of a microbial…
A: An antimicrobial therapy kills or represses the development of microorganisms, for example,…
Q: This test gives yellow solution, indicating the presence of proline* A. Biuret B. Ninhydrin C.…
A: Different of the tests mentioned here are tests for detection of amino acids. In the next step we…
Q: Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 10…
A: Cell count: The cell count data is generally utilized to predict the number of living microbes…
Q: Use the differential test matrix for this question. You are performing a single unknown. You have…
A: Methyl red test is as also called as MR test. This test is used to determine if the organism…
Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…
A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…
Q: Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) O Sodium…
A: A major goal of the nucleic acid isolation is the removal of proteins . The seperation of nucleic…
Q: hypothetical
A: Isolation and characterization of plant and human pathogenic bacteria from green pepper (Capsicum…
Q: ) Gel filtration chromatography on Sephadex G50 b.SDS-PAGE
A: Gel filtration: with this method we could separate protein with respect to molecular weight in the…
Q: What is the use of color reaction tests for proteins in diagnosing diseases? Give an example too!
A: Diagnostics, which can diagnose illness, disorders, or infections, is quickly becoming a critical…
Q: Please explain the theoretical background (positive color reactions and what is being detected)…
A: Proteins are the polymers of amino acids. The amino acids are joined together by the peptide bonds.…
Q: You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the…
A:
Q: The VP test is a confirmatory test. In what situations would this test be utilized?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: Of the two principal polymers used in column chromatography and electrophoresis, which one would be…
A: Column chromatography is a purification method that uses the polarity of compounds for the…
Q: Which of the following antibiotics exhibit the greatest antibacterial efficacy in the Kirby-Bauer…
A: According to Bartleby guidelines, we are supposed to attempt first question in case of multiple…
Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…
A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…
Q: What is the importance of performing the Modified Allen Test? Give at least three.
A: Modified Allen test: This test is done to assess the integrity of the ulnar artery, To perform this…
Q: One of the following matches indicatIng early microbial spoilage of pharmaceutical products is…
A: Microorganisms are tiny unicellular organisms that cannot be observed by unaided eyes. They may be…
Q: Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.
A:
Q: Describe three problems associated with using the standard plate count method for determining the…
A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…
Q: You perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa.…
A: ELISA or enzyme-linked immunosorbent assay is a Molecular Biology technique used to detect and…
Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…
A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…
Q: Which test require a straight line inoculation? Choose all that apply. A) Bile esculinase B) NaCl…
A: Inoculation is a form of immunisation that involves the introduction of an infectious material onto…
Q: You have a culture of bacteria with an unknown number of bacteria in it. You have at your disposal…
A: Dilutions of microbial cultures or clinical and environmental samples are regularly needed in the…
Q: The following results were obtained from a disk diffusion test for microbial susceptibility to…
A: Zone of inhibition of an antibiotic shows its effectiveness. It is termed as the area or space where…
Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…
A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…
Q: A measurement of a microbe is reported as 1 × 10-6 m, also known as centimeters (cm). millimeters…
A: Microbes are organisms that cannot be seen with naked eyes. They can be seen only with the help of a…
Q: Examine the given table below. What interpretation can be drawn from the data regarding the test…
A: given here is a table that depicts phenol and a test disinfectant at various dilutions, following is…
Q: The first step in identifying an unknown bacterium is to __________. culture your unknown on…
A: Bacteria are the minute microorganisms that are unicellular and have a cellular level of…
Q: Uses of tannins consist of: Select one: a. leather tanning and ink manufacturing b. Astringent,…
A: Tannins: Tannin is also called as tannic acid that acts as a deterrents and is used for various…
Q: This test gives red color, indicating the presence of cysteine.* A. Lead acetate test B.…
A: Cystein is a sulphur containing amino acid that is capable of forming disulphide bridges in the…
Q: UNKNOWN DNP test Tollens Benedicts + orange solid RESULT Silver mirror red precipitate
A: Functional groups are chemical motifs, that have a common function. The most important functional…
Q: Discuss the functions of the following components in performing gel electrophoresis;…
A: Introduction: The technique of gel electrophoresis is used to separate DNA fragments based on their…
Q: A serial dilution of a bacterial culture yields the following number of colonies. Which plate(s)…
A: Serial dilution is used to estimate thr number of bacterial cells present in a sample to reduce a…
Q: what do the colors in the kirby bauer plate represent black/dark area = tan/yellow are small…
A: KIRBY BAUER METHOD- Antibiotic sensitivity test is performed by using the agar diffusion method in…
Q: You carry out a dilution adding 20 mL of solution A and 80 mL of water. This dilution can NOT be…
A: In laboratory, various concentration of substances are needed for different experiments. In some…
Q: cationic microbacteriaI peptide is anaIysed using cation exchange chromatography where the packing…
A: Cation exchange chromatography uses a negatively charged ion exchange resin with an affinity for…
Q: Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid. You receive a prescription for…
A: Q.1 Available dose of tetracycline - 250 mg / 5ml . Prescribed dose = 500 mg QID ( means 4 times…
Q: A urine culture had the following culture results: Sheep blood Columbia CNA MacConkey Swarming No…
A: In urine culture, MacConkey agar is used to isolate gram-negative enteric bacteria and also…
Q: Would any of the above give a positive Lieberman-Burchard test? Explain your answer.
A: The endocrine system is a vital biological system that produces various hormones. These hormones…
Monospot: inconclusive
Heterophile antibody titer of 1:56
Differential tests:
Guinea pig kidney titer of 1:56
Beef red blood cell titer of 0
How would you interpret the above results?
What other confirmatory test can be utilized to resolve doubtful?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Enzyme immunoassay tests are used to screen blood specimens for the presence of antibodies to HIV, the virus that causes AIDS. Antibodies indicate the presence of the virus. The test is quite accurate but is not always correct. Approximate probabilities of positive and negative test results when the blood tested does and does not actually contain antibodies to HIV are given in the table. Test Result Positive Negative Antibodies present 0.9985 0.0015 Antibodies absent 0.0060 0.9940 Suppose that 1%1% of a large population carries antibodies to HIV in their blood. Probabilities from trials with 2897 people known to be free of HIV antibodies and 673 people known to be infected, reported in J. Richard George, "Alternative specimen sources: methods for confirming positives," 1998 Conference on the Laboratory Science of HIV, found online at the Centers for Disease Control and Prevention, www.cdc.gov. (b) What is the probability that the test is positive for a…The VP test is a confirmatory test. In what situations would this test be utilized?List some conditions that cause false positive reactions with the RPR test. Short answer
- If the highest dilution with a color change in an ELISA test is 1:64, the antibody titer is 640. true falseYou perform an ELISA to test hospital soap samples for contamination with Pseudomonas aeruginosa. Other than the different molecule of interest, this ELISA works exactly like the one you saw in the video. You run the soap sample, a negative control, and a positive control, all in duplicate. How would you interpret the following results? Laboratory Notebook: ELISA – Pseudomonas aeruginosa Well Contents Observation at 15 min Result 1 Negative control 2 Soap sample 3 Positive control 4 Negative control 5 Soap sample 6 Positive control 7 Empty well 8 Empty well The soap is POSITIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the NEGATIVE control didn’t work. The soap is NEGATIVE for the Pseudomonas aeruginosa antigen. You cannot interpret the soap data, because the POSITIVE control didn’t work.A nine-laboratory cooperative study was performed to evaluate quality control for susceptibility tests with 30-µg netilmicin disks [6]. Each laboratory tested three standard control strains on a different lot of Mueller-Hinton agar, with 150 tests performed per laboratory. For protocol control, each laboratory also performed 15 additional tests on eachof the control strains using the same lot of Mueller-Hinton agar across laboratories. The mean zone diameters for each of the nine laboratories are given in Table 6.11. *6.20 Provide a point and interval estimate (95% CI) for the interlaboratory standard deviation of mean zone diameters for each type of control strain, assuming each laboratory uses different media to perform the susceptibility tests. *6.21 Answer Problem 6.20 assuming each laboratory uses a common medium to perform the susceptibility tests. 6.22 Are there any advantages to using a common medium versus using different media for performing the susceptibility tests with…
- List some conditions that cause false positive reactions with the RPR test?WIDAL TEST Principle: The test depends on the ability of antibody in the patient’s serum to agglutinate the stained bacterial antigens. When this occurs, the aggregates become clearly visible to the naked eye. Materials: Test tube 75 x 12 mm Physiological saline (0.9%) Incubator or water bath Slides Procedure: A. Rapid Slide Test Semi-Quantitative Method 1. Using a graduated pipette add the following amounts of serum to consecutive circles on a slide for each dilution under test. 0.08 ml, 0.04 ml, 0.02 ml, 0.01 ml and 0.005 ml 2. Thoroughly resuspend the antigen and add a drop to the appropriate circle on the slide. 3. Mix the drops and spread to cover the entire test circle. 4. Gently and evenly, rock and rotate the test slide for 1 minute then examine the slide for agglutination. 5. Results obtained correspond to tube agglutination titer of 1:20, 1:40, 1:80, 1:160, 1:320 respectively. 6. It is advisable to confirm a slide…If a blood sample agglutinates when mixed with Anti-A serum, but does not agglutinate when mixed with Anti-B serum, what is the blood type of that sample? Group of answer choices B A AB O
- True or false? Codes 90476-90748 identify the vaccine product only.At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate