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3. How will you prepare 500 ml of 0.750 M citrate buffer with pH of 4.25 from solid citric acid (C6H8O7) and solid sodium citrate (Na3C6H5O7). The Ka of citric acid is 7.40 x 10-4 .  Given: • (Na3C6H5O7) solid sodium citrate = conjugate base in molarity [? −] • (C6H8O7) solid citric acid = weak acid in molarity [??] • 7.40 × 10−4 ?? of citric acid = ??? (acid-dissociation constant) • 500 ml of 0.750 M citrate buffer

3. How will you prepare 500 ml of 0.750 M citrate buffer with pH of 4.25 from solid citric acid (C6H8O7) and solid sodium citrate (Na3C6H5O7). The Ka of citric acid is 7.40 x 10-4 .  Given: • (Na3C6H5O7) solid sodium citrate = conjugate base in molarity [? −] • (C6H8O7) solid citric acid = weak acid in molarity [??] • 7.40 × 10−4 ?? of citric acid = ??? (acid-dissociation constant) • 500 ml of 0.750 M citrate buffer

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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3. How will you prepare 500 ml of 0.750 M citrate buffer with pH of 4.25 from solid citric acid (C6H8O7) and solid sodium citrate (Na3C6H5O7). The Ka of citric acid is 7.40 x 10-4 . 

Given:

• (Na3C6H5O7) solid sodium citrate = conjugate base in molarity [? −]

• (C6H8O7) solid citric acid = weak acid in molarity [??]

• 7.40 × 10−4 ?? of citric acid = ??? (acid-dissociation constant)

• 500 ml of 0.750 M citrate buffer

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