3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa. Thirty six mg of this pure protein was treated with excess of fluorodinitrobenzene. After the reaction and complete acid hydrolysis the mixture was found to contain 356 µg of dinitrobenzene derivative of methionine (free acid) and no other amino acid-dinitrobenzene derivatives. Is this protein consisting of single polypeptide chain or multiple subunits? If it consists of multiple subunits, then how many? From these data, can you calculate the molecular weight of the protein more precisely?

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A: Proteins present in a solution absorb ultraviolet light having an  absorbance maxima of 280 and 200…

Q: VII. Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the…

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A: * Given that you can load 100 µg of cell extract onto a gel and can detect 10 ng in a single band by…

Q: So which form of the arginine side chain is the ionized form? And when the side chain is ionized the…

A: Arginine is an amino acid with a side chain that contains a guanidine group. In its conjugate acid…

Q: If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the…

A: The biuret assay is a colorimetric method used to quantify the concentration of proteins in a…

Q: 16. A quantitative study of the interaction of a protein with its ligand yielded the following…

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Q: Suppose you have two genetic variants of a large protein that differ only in that one contains a…

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Q: 1. Consider the peptides P-A-I-G-E and M-A-T-T. a. Draw the structure of each peptide at a pH of 7.0…

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Q: "Regarding resolution of mass spectrometers and various ways mass of peptides can be represented,…

A: Mass spectrometry is a measured charge to mass ratio.

Q: 7. A protein has a molecular mass of 400 kDa when measured by gel filtration. When subjected to gel…

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Q: Write down the values in bold print. You are isolating a protein with a pl of 8.5 using cation…

A: As given in the question, a protein with pI of 8.5 was isolated using cation exchange…

Q: affinity

A:

Q: ✓ Part B What further experiment should be carried out in order to determine the primary structure…

A: Introduction Protein is the most abundant macromolecule in our body.  Amino acids are the building…

Q: Part C-Cold dentauration In the lecture, we observed that the protein stability curve as described…

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Q: Which of the peptide sequences below best matches the hydropathy plot shown? 5 10 15 Residue Number…

A: Hydropathy plot is a quantitative analysis of the distribution of amino acids in a protein.…

Q: 7. Imagine you're purifying a protein with a pl of 6.5 that has a 6-Histidine tag from E. coli…

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Q: What is the function of sodium dodecyl sulfate (SDS) in SDS-PAGE? stabilizes the gel matrix,…

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Q: (a) 1 Normalized fluorescence 0.8 0.6 0.4 0.2 0 50 55 OM 0.100 M 0.200 M 0.300 M 0.500 M 1.00 M 2.00…

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Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

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Q: You perform electrophoresis of 3 proteins, Hemoglobin (pl: 6.8), DNA Polymerase (pl: 5.2) and…

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Q: 1.0 0.9 Z 0.8 15. A pharmaceutical company studied the binding of three different compounds, X, Y,…

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Q: Describe a two-step purification procedure that could be used to purify/isolate protein A from the…

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Q: detail how cation exchange chromatography works and what you would use to elute your target protein.…

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Q: A protein was completely digested with trypsin and an internal peptide (i.e. not derived from the N-…

A: Amino Acid analysis or Protein Sequencing is a common method used to determine the sequence of amino…

Q: You gathered your data from Lab 4, and found the following values: Total Activity of lysozyme in…

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Q: Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels. 1. A…

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Q: Analysis of a peptide antibiotic purified from a strain of Bacillus brevii resulted in the following…

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Q: Which of the following deoxyoligonucleotides will hybridize with the following sequence 5' AGACTGGTC…

A: Deoxyoligonucleotides are short strands of DNA that are composed of a sequence of…

Q: Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations…

A: The Bradford protein assay is a quick and accurate spectroscopic analytical procedure used to…

Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.