2b)An ancient gene underwent duplication during the course of evolution to yield two chain genes designated as δ and ε genes. Jessica’s final year project deals with the metagenomic studies of anfH homologous genes from freshwater lake samples in Kampar Eastlake. She successfully cloned and sequenced two partial anfH homologous gene sequences. BlastX analyses revealed that one of the DNA sequences showed highest similarity to AnfH protein from a Brazilian soil bacteria isolate while the second sequence showed highest identity to the AnfH protein sequence of a bacterium that was isolated from Tonle Sap, a freshwater lake in Cambodia. To determine their evolutionary relationship, she subsequently constructed a phylogenetic tree using the corresponding protein sequences. Describe the steps that are required to perform the analysis.
2b)An ancient gene underwent duplication during the course of evolution to yield two chain genes designated as δ and ε genes. Jessica’s final year project deals with the metagenomic studies of anfH homologous genes from freshwater lake samples in Kampar Eastlake. She successfully cloned and sequenced two partial anfH homologous gene sequences.
BlastX analyses revealed that one of the DNA sequences showed highest
similarity to AnfH protein from a Brazilian soil bacteria isolate while the second sequence showed highest identity to the AnfH protein sequence of a bacterium that was isolated from Tonle Sap, a freshwater lake in Cambodia. To determine their evolutionary relationship, she subsequently constructed a phylogenetic tree using the corresponding protein sequences. Describe the steps that are required to
perform the analysis.
A phylogenetic tree is a branching diagram or tree that depicts the evolutionary relationships among distinct biological species or other entities based on physical or genetic similarities and differences. All life on Earth is represented by a single phylogenetic tree, showing that they share a common ancestor. It's a graphic that shows the evolutionary ancestry of various species, creatures, or genes from a common ancestor.
The method of estimating evolutionary relationships is phylogenetic analysis. The sequence of a common gene or protein can be utilized to determine the evolutionary relationship of species in molecular phylogenetic analysis.
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