2a)You are provided with in vitro plantlets of a plant species. Explain how you would isolate protoplasts and initiate cell suspension cultures using the provided plantlet cultures

Q: What is the function of food vocoules?

A: Amoeba and Paramecium are the unicellular organisms that belong to the Protista family. Their cell…

Q: Find out what the various components of the medium used for propagation of an explant in vitro are?

A: Plant tissue culture is a technique through which plant cells, tissues or plant parts are grown on…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: Differential centrifugation can be improved through the use of a density gradient. What is the…

A: In scientific experiments, various techniques are used to isolate, measure or quantify samples based…

Q: During the Lederberg and Tatum experiment, theresearchers compared the growth of mutant strains…

A: Conjugation is the process by which bacteria transfers its genetic material to the other bacteria by…

Q: what tests can help  to differentiate Pseudomonads aeroginosa, Pseudomonads fluorescents, and…

A: The Gram-negative bacteria have an outer membrane that protects them from various antibiotics. The…

Q: Discuss the role of the addition of ammonium chloride potassium lysius buffer in splenocyte culture…

A: Any form of white blood cell that is found in the spleen or has been separated from splenic tissue…

Q: Macro and micro elements

A: macro-nutrients required in comparatively larger amounts such as Nitrogen , Phosphorus , Potash ,…

Q: inical application: A 44-year-old man with HIV is receiving antibiotics through a intravenous…

A: HIV or human immunodeficiency virus is a virus causes AIDS in humans. HIV contains single stranded…

Q: How can we steralize Animal cell in culture?

A: Sterlisation is the process of removal of any microorganisms present in vegetative or spore form.…

Q: Why might it be difi cult to develop a manufacturing cell?

A: Cell manufacturing is the process that requires a large-scale expansion of cells without…

Q: What is a Conditioned cell-free medium(CCFM)?

A: Culture medium is a liquid or gel designed to support the growth of microorganisms.  There are…

Q: Cor the veneral formula of exponentia! grovth, where N is 2 and raised to the n pow.et, ne cell will…

A: There are multiple phases in cell growth: Lag phase: The cells prepare themselves for cell…

Q: TGF-beta treatment cause cells to grow more or less in the soft-agar assay

A: TGF beta The transforming growth factor-beta is a cytokine a protein which is produced by all types…

Q: Discuss the flaws that should be identified and addressed throughout the cell morphology method's…

A: Cell morphology describes size, shape and structure of particular cell.

Q: Decide whether each of these statements is true or false, and then explain why. Laser-capture…

A: Different techniques are available to visualise a single cell from a given tissue.

Q: Why must materials and instruments used in tissue culture be kept sterile?

A: Sterilization of explants is a key step in plant tissue culture.

Q: How is a cells suspension culture made?

A: Answer: Cell suspension culture is the process of replicating single cells more quickly in a liquid…

Q: 1.What colony characterisitics can be used for differentiation of bacterial species? As an example,…

A: Bacteria are prokaryotic microscopic organisms that have different characteristics. Their…

Q: What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating")…

A: Quadrant streak method is used for isolating the bacteria based on their differences. Four Agar…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: Describe how Blue/White screening reagent helps in screening the colonies of transformed cells.

A: The bacteria are a microscopic small organism. They are cultured in laboratories for many purposes.…

Q: Besides the cheek cells, what other cells might you see on the specimen? Consider both the structure…

A: Cheek cells are the epithelial cells lining the oral cavity. These are eukayotic cells with a…

Q: Nar ang be detail three (3) disadvantages of differential

A: Differential centrifugation is used to separate various organic lease and subcellular organelles of…

Q: Describe a technique that will allow you to separate cells from media

A: ANSWER) There are various techniques which are used to separate the cells from the medium. Some of…

Q: 3) If Hybridoma cells are cultured supported in an agarose matrix in which nutrients and oxygen are…

A: A hybrid cell utilized as the purpose for the generation of antibodies in huge quantities for…

Q: The death of Geobacillus stearothermophilus spores is routinely used to assess the effectiveness of…

A: Sterilization procedures are often assessed by looking its ability to kill Bacillus endospores.…

Q: A common step in the isolation of cells from asample of animal tissue is to treat the tissue with…

A: A combination of trypsin and EDTA is often used to dissociate cells and tissues. Trypsin is a…

Q: https://studylib.net/doc/8245959/lab-7--got-milk%3F follow and open the link then answer the…

A: Introduction  Operon model gives the basic concept about the gene regulation in bacteria. It was…

Q: Describe the working principles of a method used to test the viability of protoplasts based on…

A: Protoplasts are cells that undergo enzymatic action to remove their cell wall. Protoplasts can be…

Q: why do we used Living cell microsope for?

A: Microscope is an instrument that is used to observe objects that are too small to be seen by our…

Q: Discuss the principle involved with the use of a centrifuge. 2. Enumerate some specific examples of…

A: Various techniques are used for isolating as well as testing the substances present in a mixture.…

Q: Descibe shortly four factors which may, as a result of heat generation, have an effect on the…

A: Electrophoresis is the technique used in the laboratory to separate DNA, RNA, or protein molecules…

Q: (a) What do reverse transcriptases do? Give two examples of these and what they do in the cell.…

A: Recombinant DNA technology involves the insertion of a transgene into a microorganism. Polymerase…

Q: What are the basic differences from a primary cell culture, cell line and a cell strain? 2.…

A: Answer 1: - Primary cell culture is defined as: - an ex vivo culture of cells freshly obtained from…

Q: What is the name of the technique that create this graph

A: The graph appears to be showing results obtained for a growing cell population when DNA content of…

Q: Time (min) 0 15 30 45 60 75 90 No drug 0.183 0.232 0.536 0. 746 0.892 1.068 1.150 Absorbance (A540…

A: The whole question relates to drug susceptibility test that is important in order to determine…

Q: Briefly explain the mechanism behind competent cell preparation by chemical treatment and chemical…

A: The competent cells are the bacterial cells that can take up foreign DNA from the surrounding by…

Q: Comparing the different methods for counting bacterial cells, which of the following is FALSE? A.…

A: Prokaryotes are those organisms which lack a membrane bound nucleus. They consist of eubacteria and…

Q: Discuss, in details, the characteristics and function of the unique feature which enables the…

A: The onion is a bulbous plant in the Allium family that is widely used as a vegetable. Onion cells…