First LetterAGUс22. Using the provided "Genetic Code-Reference" answer the following question.Based on the following DNA template strand, write out the amino acid chain produced.23. Consider the following mRNA base codon sequence 5'-AUC-GAA-3' and the provided "GeneticCode-Reference".Genetic Code-ReferenceUUUUUCUUAUUGCUUCUCCUACUG(mutated or silent)(mutated or silent)b. Briefly explain your reasoning for each. (be sure to include both parts)AULLYAUUa. Label which of the following would result in a mutated amino acid sequence or a silentmutation. (May help to first determine the original amino acid sequence, then compareto mutations)UPhemRNA codon sequence:anticodon sequence:amino acid sequence:LeuLeu5'-AUA-GAA-3'Val5'- AUC-GAC-3'AUC IlleAUAAUAJ*AUG Met/StartGUUGUCGUAGUGUCUUCCUCAUCG)CCU)CCCcccCCA000CCGACUACUACCACAACG,CGCUGCCGCAGCGSecond LetterSerProThr3'-CAA-GTC-TGT-5'AlaUAUUAC) TyrТугA**UAA StopUAG StopCAU]CACJCAACAG/GinGAUGACGAAGAGAAU AstAAC)AAA]AAG/Lys>HisAspGluGUGUUGC Cys**UGA StopUGG TrpCGUCGCCGACGGArgAGUAGCAGA]AGG ArgGGUGGCGGAGGGSerGlyThird LetterUс сAGUсAAG11UCAGUCAG

Transcription is the process of formation of RNA from a DNA. The RNA chain is synthesized in the…

Answered: 22. Using the provided "Genetic…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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25. A portion of an mRNA attached to a ribosome reads: 5′ GACAUGAACAGC 3′ If a tRNA with a methionine amino acid attached is in the P site of the ribosome, a tRNA with which amino acid attached will enter the A site? Group of answer choices 1. Asparagine 2. Lysine 3. Threonine 4. Glutamic Acid
1. Translation a)Explain the role of ribosomes in the process of protein translation.b. Explain the role of tRNA molecules in protein translation.c. Define the following terms:1. codon 2. anticodon. Explain how a codon is used in the process of translation.
1. What is the length in AA’s of the LilP protein? Assume fMet is NOT CLEAVED. 2. Write out the sequence of the polypeptide in AA: use the three letter notation, e.g. Met-Ser-Pro-
2. H. Gobind Khorana deciphered the genetic code by (1) chemically synthesizing DNA molecules of known sequence, (2) using this synthetic DNA as a template to produce RNA in a reaction involving RNA polymerase and dNTPs, then finally (3) using this RNA in an in vitro translation experiment with the ribosome and amino acyl-tRNA (aa-tRNA) building blocks to produce peptides. For each single-stranded deoxyribonucleotide sequence Khorana synthesized (shown below), a single polypeptide was produced in the in vitro translation step (3): (1) Synthetic DNA d(TTT)n d(CCC)n d(AAA)n d(GGG)n d(AAACCC)n d(AC)n d(ACA)n a. (2) RNA encoded by synthetic DNA (3) Peptide product composition polylysine polyglycine polyphenylalanine polyproline Equal F and G Equal C and V polycysteine Fill in the RNA sequences that were produced by RNA polymerase in column (2). b. When Khorana conducted this work, one looming question in the field was whether or not the genetic code is overlapping or non-overlapping. In a…
1e) Give the sequence of every codon this tRNA, with the anticodon 5'AGG3', could base pair with (perfect and wobble matches), and name the amino acid coded for by each codon whose sequence you have written.
15)
A segment of template DNA is known to contain the following base sequence: 3' GATACCTTTGTGTAGTCATCTT 5' a) Write the mRNA that would be transcribed from this DNA fragment. b) Highlight the starter and the stopper in your mRNA sequence. Write the sequence of amino acids which would be encoded in translation. asap please
1. In what direction does a polymerase move when synthesizing a strand of mRNA? Briefly Explain. 2. Instead of the term “Formation of a nucleoside”, what could the name of the reaction be? What functional group is being formed? Briefly Explain.
1. Given the DNA sequence below: 5’-ACATGTGTACAGGCTTTGTCTGAATGGCTT-3’ 3’-TGTACACATGTCCGAAACAGACTTACCGAA-5’ Translate the mRNA. (Using the 3-letter code for amino acids, write the primary structure of the peptide that will be produced.) 2. Given the DNA sequence below: 5’-ACATGTGTACAGGCTTTGTCTGAATGGCTT-3’ 3’-TGTACACATGTCCGAAACAGACTTACCGAA-5’ Translate the mRNA. (Using the 3-letter code for amino acids, write the primary structure of the peptide that will be produced.)
41. The following 9 TRNAS have anticodon loop regions that base pair with the mRNA in the ribosome. Using your knowledge of tRNA-MRNA base pairing and the genetic code chart (+lecture slides), indicate the MRNA codon message being read by the ribosome as well as the1-letter amino acids that is generated. Anticodon Anticodon Anticodon Anticodon 4 Anticodon Anticodon 6. 2 5'-IAG-3' 5'-UAU-3' 5'-АAА-3' 5'-CUC-3' 5'-GAU-3' 5'-AGA-3' codon MRNA 5'-__-3' 5' 3' 5' 3' 5' 3' 5' 3' 5' 3' 1 letter amino acid Anticodon Anticodon Anticodon 8 7 5'-CCG-3' 5'-GUU-3' 5'-CGC-3' codon 5'-__-3' 5' 3' 5' _3' mRNA 1 letter amino acid
36.Start with two exons and an intervening intron. Include the cap and poly-A tail in your starting pre-mRNA. Show 2’-OH, 3’-OH, O-P-O phosphodiester bonds for the two transesterification reactions that splice exon 1 and exon 2. Be sure to include the branchpoint A and intron consensus sequences. You will need to show where the incoming -OH attacks the O-P-O bond to allow correct splicing in the lariat and between exons. Make your arrows precise. Show the lariat with the consensus sequences. Whenever possible, show 5’ and 3’ ends. Explain the fate of the lariat after it forms?
20. TRNA stands for transfer RNA, since a tRNA molecule transfers an amino acid to the growing protein molecule. You could also think of tRNA as translation RNA. Explain how tRNA molecules help the ribosome to translate the sequence.of nucleotides in mRNA to i sequence of amino acids in a protein. To see the process of translation in action, watch "Translation" at https://www.biointeractive.org/classroom-resources/translation-basic-detail. 21a. Fill in the blanks to label each type of molecule in this figure. a.tRNA $ & 7 8 9.

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