2. You use Sanger method to sequence the OprL gene from P. aeruginosa. Part of the template DNA sequence is shown below. For clarity: Dashes indicate DNA whose sequence has been unspecified. A space is left after every 5 nucleotides. 5tatgg aaatg ctgaa attcg gctcc ----3' 3'atacc tttac gactt taagc cgagg ----5' Your 20-nucleotide long sequencing primer anneals to the highlighted sequence at the left. The primer sequence ends: ...TATGG-3'. In your sequencing reaction, ddTTP is labeled with a red fluorescent tag. When this Sanger sequencing reaction is complete, what will be the lengths, in nucleotides, of the three shortest reaction products that have the red tag? nt nt nt
2. You use Sanger method to sequence the OprL gene from P. aeruginosa. Part of the template DNA sequence is shown below. For clarity: Dashes indicate DNA whose sequence has been unspecified. A space is left after every 5 nucleotides. 5tatgg aaatg ctgaa attcg gctcc ----3' 3'atacc tttac gactt taagc cgagg ----5' Your 20-nucleotide long sequencing primer anneals to the highlighted sequence at the left. The primer sequence ends: ...TATGG-3'. In your sequencing reaction, ddTTP is labeled with a red fluorescent tag. When this Sanger sequencing reaction is complete, what will be the lengths, in nucleotides, of the three shortest reaction products that have the red tag? nt nt nt
Chapter2: Mathematics For Microeconomics
Section: Chapter Questions
Problem 2.16P
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Transcribed Image Text:2. You use Sanger method to sequence the OprL gene from P. aeruginosa. Part of the
template DNA sequence is shown below. For clarity: Dashes indicate DNA whose
sequence has been unspecified. A space is left after every 5 nucleotides.
5tatgg aaatg ctgaa attcg gctcc ----3'
3'atacc tttac gactt taagc cgagg ----5'
Your 20-nucleotide long sequencing primer anneals to the highlighted sequence at the left. The
primer sequence ends: ...TATGG-3'.
In your sequencing reaction, ddTTP is labeled with a red fluorescent tag. When this Sanger
sequencing reaction is complete, what will be the lengths, in nucleotides, of the three shortest
reaction products that have the red tag?
nt
nt
nt
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