2. The kinetics of an enzyme are measured as a function of substrate concentration in the presence and absence of 100 uM inhibitor. Show calculations, include the table of values needed to make your graphs and provide full explanations for each of the following. Velocity (UM/minute) [S] (UM) No inhibitor Inhibitor 3 10.4 2.1 5 14.5 2.9 10 22.5 4.5 30 33.8 6.8 90 40.5 8.1 i) Calculate the values of Km and Vmax in the absence of the inhibitor? Show all calculations. Circle your final answers. Make sure units are included II) What are the values of Km and Vmax in the presence of the inhibitor? Show all calculations. Circle your final answers. Make sure units are included. iii) What type of inhibition is this? Explain your reasoning
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
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