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- Calculation for a neutral red assay please include steps since it is essential for the report and it is marked.Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I only8. What is the best recipe for an LDH enzyme activity assay?
- Discuss the principles of Thiobarbituric reactive species (BARS) assay1. What is the purpose of the different reagents used in the procedure of kato-katz technique? 2. How are the Kato Katz Technique results reported? 3. What are the advantages and disadvantages of using Kato-Katz Technique?For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.
- 1. What is the significance of the formation of the colored product in the oxidase test? Answer this comprehensively and please, do not just copy from somewhere.This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075 Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8 6 43.0 44.9 9 42.6 45.2 12 43.5 45.6 15 44.0 45.7 18 43.8 45.9 21 44.0 46.4 24 44.1 46.5 27 43.9 46.7 30 43.7 46.9 43.61.Most enzymatic reactions consume substrate or produce products which cannot be monitored directly with spectrophotometric methods because they do not absorb light. What can be done in this case to monitor the substrate consumption or product formation in order to calculate kinetic parameters? switch to a continuous assay switch to a discontinuous assay use a coupled reaction which converts the product into one that has a chromophore All of the above
- Match the following methods of Anlaysis v Fractional analysis, methylation, and periodate oxidation No Match v Use of endo- and exoglycosidases B. Enzymatic Method v Polarimetry, mass spectrometry, & NMR C. Chemical Method v Chromatographic separations of hydrolyzed fragments .Spectroscopic1. Give the importance of carbohydrate fermentation test in biochemical testing and enumerate the enzymes that are involved. 2. What are the substances added in the culture media utilized by the organism producing hydrogen sulfide? Give 2 other media used for the production of hydrogen sulfide. 3. Explain the mechanism taking place in the hydrolysis of urea which leads to the formation of bluish red color. 4. Discuss why human blood plasma will not always yield reliable results.A 1/10 dilution of enzyme extract has been done for the protein assay. The experimental results are given below. Tube Phosphate buffer 0.1 M pH 7 (µL) Diluted enzyme extract (uL) Bradford reagent (mL) DO at 595 nm 0,8 0,7 0,6 0,5 0,4 0,3 0,2 0,1 A595 0- 0 1 50 50 1 0.102 1- Calculate the mass of proteins contained in 50 or 100 μL of diluted extract thanks to the calibration curve (graph below), performed with serum albumin (SAB). 2- Calculate the protein concentration of undiluted enzyme extract. 2 3 5 50 50 0 50 50 100 100 1 1 1 1 0.098 0.101 0.199 0.200 2 6 8 10 SAB (ug per tube) Data: the slope of the calibration curve is 0,05 µg¹¹. 4 0 12 14 6 0 100 1 0.200 16